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Revealing the role of CCoAOMT1: fine-tuning bHLH transcription factors for optimal anther development

文献类型： 外文期刊

作者： Lai, Zesen 1 ; Wang, Jianzheng 1 ; Fu, Ying 1 ; Wang, Menghan 1 ; Ma, Hong 5 ; Peng, Shiqing 3 ; Chang, Fang 1 ;

作者机构： 1.Fudan Univ, Inst Plant Biol, Key Lab Biodivers Sci & Ecol Engn, State Key Lab Genet Engn,Minist Educ,Sch Life Sci, Shanghai 200438, Peoples R China

2.Fudan Univ, Inst Biodivers Sci, Inst Plant Biol, Sch Life Sci, Shanghai 200438, Peoples R China

3.Hainan Univ, Sch Trop Agr & Forestry, Agr Rural Affairs & Rural Revitalizat, Haikou 570228, Peoples R China

4.Chinese Acad Trop Agr Sci, Inst Trop Biosci & Biotechnol, Key Lab Biol & Genet Resources Trop Crops, Minist Agr, Haikou 571101, Peoples R China

5.Penn State Univ, Huck Inst Life Sci, Dept Biol, University Pk, PA 16802 USA

关键词： anther development; nuclear localization; bHLH; O-methyltransferase; transcription factor homeostasis

期刊名称：SCIENCE CHINA-LIFE SCIENCES （ 影响因子：9.1； 五年影响因子：7.1 ）

ISSN： 1674-7305

年卷期： 2024 年 67 卷 3 期

页码：

收录情况： SCI

摘要： The tapetum, a crucial innermost layer encompassing male reproductive cells within the anther wall, plays a pivotal role in normal pollen development. The transcription factors (TFs) bHLH010/089/091 redundantly facilitate the rapid nuclear accumulation of DYSFUNCTIONAL TAPETUM 1, a gatekeeper TF in the tapetum. Nevertheless, the regulatory mechanisms governing the activity of bHLH010/089/091 remain unknown. In this study, we reveal that caffeoyl coenzyme A O-methyltransferase 1 (CCoAOMT1) is a negative regulator affecting the nuclear localization and function of bHLH010 and bHLH089, probably through their K259 site. Our findings underscore that CCoAOMT1 promotes the nuclear export and degradation of bHLH010 and bHLH089. Intriguingly, elevated CCoAOMT1 expression resulted in defective pollen development, mirroring the phenotype observed in bhlh010 bhlh089 mutants. Moreover, our investigation revealed that the K259A mutation in the bHLH089 protein disrupted its translocation from the nucleus to the cytosol and impeded its degradation induced by CCoAOMT1. Importantly, transgenic plants with the probHLH089::bHLH089K259A construct failed to rescue proper pollen development or gene expression in bhlh010 bhlh089 mutants. Collectively, these findings emphasize the need to maintain balanced TF homeostasis for male fertility. They firmly establish CCoAOMT1 as a pivotal regulator that is instrumental in achieving equilibrium between the induction of the tapetum transcriptional network and ensuring appropriate anther development.

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