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Rapid and sensitive detection of African swine fever virus in pork using recombinase aided amplification combined with QDMs-based test strip

文献类型： 外文期刊

作者： Wen, Xiaoyun 1 ; Xie, Qingyun 2 ; Li, Jiahao 3 ; Pei, Yanrui 3 ; Bai, Yun 2 ; Liu, Fei 3 ; Cui, Haiying 1 ; Shao, Guoqing 2 ; Feng, Zhixin 2 ;

作者机构： 1.Jiangsu Univ, Sch Food & Biol Engn, Zhenjiang 212013, Jiangsu, Peoples R China

2.Minist Agr, Jiangsu Acad Agr Sci, Key Lab Vet Biol Engn & Technol, Inst Vet Med, Nanjing 210014, Peoples R China

3.Nanjing Agr Univ, Joint Int Res Lab Anim Hlth & Food Safety, Minist Educ, Nanjing 210095, Jiangsu, Peoples R China

4.Nanjing Agr Univ, Single Mol Nanometry Lab Sinmolab, Nanjing 210095, Jiangsu, Peoples R China

关键词： On-site ASFV detection; Pork samples; Recombinase aided amplification (RAA); Quantum dot microspheres (QDMs)-based test strip

期刊名称：ANALYTICAL AND BIOANALYTICAL CHEMISTRY （ 影响因子：4.478； 五年影响因子：4.29 ）

ISSN： 1618-2642

年卷期： 2022 年 414 卷 13 期

页码：

收录情况： SCI

摘要： African swine fever virus (ASFV) is the pathogen of African swine fever, a highly contagious and fatal disease of wild boar and domestic pigs. The flow of ASFV through pork products is more concealed, higher risky, and more difficult to prevent and control. Presently, on-site ASFV detection methods in preclinical infected pigs and circulated pork products are lacking. Here, fluorescent test strip-based rapid ASFV detection method in pork was established combined with recombinase aided amplification (RAA) and quantum dot microspheres (QDMs). This method is specific to ASFV with no cross-reactivity to pseudorabies virus (PRV), porcine circovirus type 2 (PCV2), and porcine reproductive and respiratory syndrome virus (PRRSV). The method also showed highly sensitivity with a detection limit of 1 copy for ASFV plasmid templates containing B646L gene and 100 copies/g for DNA extracts from clinical pork samples within a short detection time of less than 25 min. Additionally, the method showed 99.17% consistency with real-time PCR in the ASFV detection of 120 clinical pork samples. Overall, the QDMs-based test strip method provides specific, sensitive, rapid, and simple detection of ASFV in pork, which may contribute to maintain the food safety of pork products, and facilitate ASFV traceability and prevention.

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