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Overexpression of 4-hydroxyphenylpyruvate dioxygenase (IbHPPD) increases abiotic stress tolerance in transgenic sweetpotato plants

文献类型： 外文期刊

作者： Kim, So-Eun 1 ; Bian, Xiaofeng 3 ; Lee, Chan-Ju 1 ; Park, Sul-U 1 ; Lim, Ye-Hoon 1 ; Kim, Beg Hab 1 ; Park, Woo Sung 4 ;

作者机构： 1.Korea Res Inst Biosci & Biotechnol KRIBB, Plant Syst Engn Res Ctr, 125 Gwahak Ro, Daejeon 34141, South Korea

2.Univ Sci & Technol UST, KRIBB Sch Biotechnol, Dept Environm Biotechnol, 217 Gajeong Ro, Daejeon 34113, South Korea

3.Jiangsu Acad Agr Sci, Inst Food Crops, Prov Key Lab Agrobiol, Nanjing 210000, Jiangsu, Peoples R China

4.Gyeongsang Natl Univ, Coll Pharm, 501 Jinjudae Ro, Jinju 52828, South Korea

5.Gyeongsang Natl Univ, Res Inst Pharmaceut Sci, 501 Jinjudae Ro, Jinju 52828, South Korea

6.Genolution Inc, R&D Ctr, 11,Beobwon Ro 11 Gil, Seoul 05836, South Korea

关键词： Sweetpotato; IbHPPD; Tocopherol; Herbicide stress; Abiotic stress

期刊名称：PLANT PHYSIOLOGY AND BIOCHEMISTRY （ 影响因子：4.27； 五年影响因子：4.816 ）

ISSN： 0981-9428

年卷期： 2021 年 167 卷

页码：

收录情况： SCI

摘要： Tocopherols are lipid-soluble compounds regarded as vitamin E compounds and they function as antioxidants in scavenging lipid peroxyl radicals and quenching reactive oxygen species (ROS). In our previous studies, we isolated five tocopherol biosynthesis genes from sweetpotato (Ipomoea batatas [L.] Lam) plants including 4hydroxyphenylpyruvate dioxygenase (IbHPPD). HPPD is the first regulatory enzyme in vitamin E biosynthesis and serves to catalyze in the first steps a-tocopherol and plastoquinone biosynthesis by converting 4-hydroxyphenylpyruvate (HPP) to homogentisic acid (HGA). In this study, we generated transgenic sweetpotato plants overexpressing IbHPPD under the control of cauliflower mosaic virus (CaMV) 35S promoter (referred to as HP plants) via Agrobacterium-mediated transformation to understand the function of IbHPPD in sweetpotato. Three transgenic lines (HP3, HP14 and HP15) with high transcript levels of IbHPPD were selected for further characterization. Compared with non-transgenic (NT) plants, HP plants exhibited enhanced tolerance to multiple environmental stresses, including salt, drought, and oxidative stresses. In addition, HP plants showed increased tolerance to the herbicide sulcotrione, which is involved in the inhibition of the HPPD. Interestingly, after stress treatments, HP plants also showed higher abscisic acid (ABA) contents than NT plants. Under dehydrated condition, HP plants displayed an elevated alpha-tocopherol content to 19-27% in leaves compared with NT plants. These results indicate that increased abiotic stress tolerance in HP plants is related to inducing enhancement of alpha-tocopherol and ABA contents.

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