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Characterization of the fatty acid binding protein 3 (FABP3) promoter and its transcriptional regulation by cAMP response element binding protein 1 (CREB1) in goat mammary epithelial cells

文献类型： 外文期刊

作者： Yao, Dawei 1 ; Zhao, Xin 1 ; Zhao, Shuying 1 ; Shi, Hengbo 4 ; Ma, Yi 1 ; Li, Jun 5 ;

作者机构： 1.Tianjin Acad Agr Sci, Tianjin Inst Anim Husb & Vet Med, Tianjin 300381, Peoples R China

2.Nankai Univ, Coll Life Sci, Tianjin, Peoples R China

3.Tianjin Agr Univ, Coll Anim Sci & Anim Med, Tianjin, Peoples R China

4.Zhejiang Univ, Coll Anim Sci, Hangzhou, Peoples R China

5.Henan Univ Anim Husb & Econ, Coll Anim Sci & Technol, Zhengzhou 450046, Peoples R China

关键词： Fatty acid binding protein 3 (FABP3) promoter; CREB1; transcriptional regulation; dairy goat

期刊名称：ANIMAL BIOTECHNOLOGY （ 影响因子：2.141； 五年影响因子：1.92 ）

ISSN： 1049-5398

年卷期：

页码：

收录情况： SCI

摘要： Fatty acid binding protein 3 (FABP3) is involved in signal transduction pathways, and in the uptake and utilization of long-chain fatty acids. However, the transcriptional regulation of FABP3 in goat is unclear. In this study, the FABP3 5 ' flanking region was amplified from goat (Capra hircus) genomic DNA. Luciferase reporter vectors containing promoter fragments of five different lengths were constructed and transfected into dairy goat mammary epithelial cells. The region of the promoter located between -1801 and -166 bp upstream of the transcription start site (TSS) exhibited the highest luciferase activity, and contained two cAMP response elements (CREs) located at -1632 bp and -189 bp. Interference with CREB1 significantly downregulated FABP3 promoter activity. In addition, FABP3 promoter activity was significantly reduced after mutation of the CRE1 (-1632 bp) and CRE2 (-189 bp) sites. Further analysis indicated that the CRE2 site was essential for the transcriptional activity induced by CREB1. These results demonstrated that CREB1 is involved in the transcriptional regulation of FABP3 expression in the goat mammary gland via a direct mechanism, thus revealing a novel signaling pathway involved in fatty acid metabolism in goat.

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