您好，欢迎访问江苏省农业科学院 机构知识库！
江苏省农业科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

The FaFlbA mutant of Fusarium asiaticum is significantly increased in nivalenol production

文献类型： 外文期刊

作者： Fang, Xin 1 ; Dong, Fei 1 ; Wang, Shuang 1 ; Wang, Gang 1 ; Wu, Deliang 1 ; Lee, Yin-Won 1 ; Mohamed, Sherif Ramzy 4 ; Goda, Amira Abdel-Karim 4 ; Xu, Jianhong 1 ; Shi, Jianrong 1 ; Liu, Xin 1 ;

作者机构： 1.Jiangsu Acad Agr Sci, Inst Food Safety & Nutr,Minist Sci & Technol,Key, Collaborat Innovat Ctr Modern Grain Circulat & Sa, Jiangsu Key Lab Food Qual & Safety,State Key Lab, Nanjing, Jiangsu, Peoples R China

2.Jiangsu Univ, Sch Food & Biol Engn, Zhenjiang, Jiangsu, Peoples R China

3.Seoul Natl Univ, Sch Agr Biotechnol, Seoul, South Korea

4.Natl Res Ctr, Food Toxicol & Contaminants Dept, Giza, Egypt

关键词： FaFlbA; Fusarium asiaticum; gene deletion; high production; nivalenol

期刊名称：JOURNAL OF APPLIED MICROBIOLOGY （ 影响因子：4.059； 五年影响因子：4.439 ）

ISSN： 1364-5072

年卷期： 2022 年 132 卷 4 期

页码：

收录情况： SCI

摘要： Aims Cereals contaminated with type B trichothecene nivalenol (NIV) and its acetylated derivative 4-acetyl-nivalenol (4-AcNIV) are a global mycotoxicological problem threatening the health of humans and livestock. Toxicological studies, quantitative determinations and screening for biodegrading micro-organisms require massive amounts of pure toxins. However, the low yield from fungal cultures and high prices of NIV and 4-AcNIV limit research progress in these areas. This work aimed to select Fusarium asiaticum mutant strains with enhanced production of NIV and 4-AcNIV. Methods and Results A total of 62 NIV-producing F. asiaticum strains were isolated and compared regarding their ability to produce NIV. Strain RR108 had the highest yield of NIV among 62 field isolates surveyed and was then genetically modified for higher production. Targeted deletion of the FaFlbA gene, encoding a regulator of G protein signalling protein, resulted in a significant increase in NIV and 4-AcNIV production in the FaFlbA deletion mutant Delta FaFlbA. The expression of three TRI genes involved in the trichothecene biosynthetic pathway was upregulated in Delta FaFlbA. Delta FaFlbA produced the highest amount of NIV and 4-AcNIV when cultured in brown long-grain rice for 21 days, and the yields were 2.07 and 2.84 g kg(-1), respectively. The mutant showed reduced fitness, including reduced conidiation, loss of perithecial development and decreased virulence on wheat heads, which makes it biologically safe for large-scale preparation and purification of NIV and 4-AcNIV. Conclusions The F. asiaticum mutant strain Delta FaFlbA presented improved production of NIV and 4-AcNIV with reduced fitness and virulence in plants. Significance and Impact of the Study Targeted deletion of the FaFlbA gene resulted in increased NIV and 4-AcNIV production. Our results provide a practical approach using genetic modification for large-scale mycotoxin production.

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：江苏省农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台