Genome editing reveals dmrt1 as an essential male sex-determining gene in Chinese tongue sole (Cynoglossus semilaevis)
文献类型： 外文期刊
作者： Cui, Zhongkai 1 ; Liu, Yun 5 ; Wang, Wenwen 1 ; Wang, Qian 1 ; Zhang, Ning 1 ; Lin, Fan 1 ; Wang, Na 1 ; Shao, Changwei 1 ;
作者机构： 1.Minist Agr, Key Lab Sustainable Dev Marine Fisheries, Qingdao 266071, Peoples R China
2.Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Qingdao 266071, Peoples R China
3.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Fisheries Sci & Food Prod Proc, Qingdao 266273, Peoples R China
4.Shanghai Ocean Univ, Coll Fisheries & Life, Shanghai 201306, Peoples R China
5.Chinese Univ Hong Kong, Sch Biomed Sci, Shatin, Hong Kong, Peoples R China
6.Chinese Univ Hong Kong, Sch Biomed Sci, Core Lab, Shenzhen Res Inst, Shenzhen 518057, Peoples R China
7.Univ Wurzburg, Physiol Chem, Biozentrum, D-97074 Wurzburg, Germany
8.Univ Clin Wurzburg, Comprehens Canc Ctr Mainfranken, D-97074 Wurzburg, Germany
9.Texas A&M Univ, Texas A&M Inst Adv Studies, College Stn, TX 77843 USA
10.Texas A&M Univ, Dept Biol, College Stn, TX 77843 USA
期刊名称：SCIENTIFIC REPORTS （ 影响因子：4.379； 五年影响因子：5.133 ）
ISSN： 2045-2322
年卷期： 2017 年 7 卷
页码：
收录情况： SCI
摘要： Chinese tongue sole is a marine fish with ZW sex determination. Genome sequencing suggested that the Z-linked dmrt1 is a putative male determination gene, but direct genetic evidence is still lacking. Here we show that TALEN of dmrt1 efficiently induced mutations of this gene. The ZZ dmrt1 mutant fish developed ovary-like testis, and the spermatogenesis was disrupted. The female-related genes foxl2 and cyp19a1a were significantly increased in the gonad of the ZZ dmrt1 mutant. Conversely, the male-related genes Sox9a and Amh were significantly decreased. The dmrt1 deficient ZZ fish grew much faster than ZZ male control. Notably, we obtained an intersex ZW fish with a testis on one side and an ovary on the other side. This fish was chimeric for a dmrt1 mutation in the ovary, and wild-type dmrt1 in the testis. Our data provide the first functional evidence that dmrt1 is a male determining gene in tongue sole.
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