Use of Microsatellite DNA Profiling to Identify Japanese Flounder, Paralichthys olivaceus of Hatchery Origin
文献类型： 外文期刊
作者： Liu, Yongxin 1 ; Yang, Runqing 1 ; Liu, Yi 2 ; Si, Fei 3 ;
作者机构： 1.Chinese Acad Fishery Sci, 150 Qingta, Beijing 100141, Peoples R China
2.CAFS, Pearl River Fisheries Res Inst, Guangzhou 510380, Guangdong, Peoples R China
3.CAFS, Beidaihe Cent Expt Stn, Qinhuangdao 066100, Peoples R China
期刊名称：JOURNAL OF THE WORLD AQUACULTURE SOCIETY （ 影响因子：2.512； 五年影响因子：2.47 ）
ISSN： 0893-8849
年卷期： 2017 年 48 卷 2 期
页码：
收录情况： SCI
摘要： Japanese flounder, Paralichthys olivaceus, is an economically important marine fish species in Asia. A suite of 18 microsatellite markers chosen from published genetic linkage maps was used to carry out parentage assignments of 188 hatchery-reared juveniles from a small number of breeders. The probabilities of exclusion for the 18 microsatellite markers were 0.604-0.913, and the effectiveness of combined probability of exclusion reached 100% when using the eight microsatellite markers with higher Excl 1 probabilities. The cultured and wild stocks (WSs) were differentiated in a release-recapture population based on these markers. Of the 321 recaptured offspring, 28.34% were assigned to their parental pairs in our broodstock, whereas the remaining offspring could not be traced back to a possible sire or dam. Significant reduction in genetic diversity of the cultured stock (CS) had not been found compared with that of the WS. The results suggest that CSs released into the wild will not adversely affect the genetic structure of natural populations. Our results demonstrate that these markers provide an efficient tool for parentage assignments and genetic analysis of Japanese flounder.
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