A portable fluorescence biosensor for rapid and sensitive glutathione detection by using quantum dots-based lateral flow test strip
文献类型： 外文期刊
作者： Liu, Juanzu 1 ; Ji, Danyang 1 ; Meng, Hongmin 1 ; Zhang, Lin 1 ; Wang, Junyan 2 ; Huang, Zhongming 1 ; Chen, Juan 1 ; Li, 1 ;
作者机构： 1.Zhengzhou Univ, Coll Chem & Mol Engn, Zhengzhou 450001, Henan, Peoples R China
2.Henan Acad Agr Sci, Inst Qual Stand & Testing Technol Agroprod, Zhengzhou 450002, Henan, Peoples R China
关键词： Lateral flow test strip; Quantum dots; Glutathione detection; Biosensor
期刊名称：SENSORS AND ACTUATORS B-CHEMICAL （ 影响因子：7.46； 五年影响因子：6.743 ）
ISSN： 0925-4005
年卷期： 2018 年 262 卷
页码：
收录情况： SCI
摘要： Glutathione (GSH) is the most abundant biothiol in cells and plays key roles in biological processes. Therefore, it is vitally important to detect GSH in biological samples. In this work, by using a novel quantum dots-based "off-on" fluorescent lateral flow test strip (LFTS), the authors describe a rapid and sensitive biosensor for point-of-care testing of GSH. In this design, bovine serum albumin (BSA) functionalized quantum dots (QDs) are chosen as signal reporter and modified on the test line of the LFTS. When Ag* binds to the surface of QDs, the fluorescence of QDs can be quenched efficiently owing to the electron transfer from QDs to Ag+. In the presence of GSH, due to the much stronger affinity between Ag* and GSH, Ag+ prefers to react with GSH to form Ag+-S bond. Consequently, the fluorescence of QDs is restored and GSH quantification is realized successfully. Under the optimal conditions, a good linear relationship from 0.1 mu A to 15 (mu)A with a detection limit of 25 nM was obtained, and the whole process can be accomplished quickly within 10 min. In addition, this proposed biosensor showed acceptable recovery (98.4%-102.2%) for the detection of GSH in Hela cells extract samples. (C) 2018 Elsevier B.V. All rights reserved.
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