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Isolation, characterization and genomic analysis of a novel lytic bacteriophage vB_SsoS-ISF002 infecting Shigella sonnei and Shigella flexneri

文献类型： 外文期刊

作者： Shahin, Khashayar 1 ; Bouzari, Majid 1 ; Wang, Ran 2 ;

作者机构： 1.Univ Isfahan, Fac Sci, Dept Biol, Hezar Jereeb St, Esfahan 8174673441, Iran

2.Jiangsu Acad Agr Sci, Inst Food Safety & Nutr, State Key Lab Cultivat Base MOST, Nanjing 210014, Jiangsu, Peoples R China

关键词： Shigellosis; Siphoviridae; T1virus; phage therapy; Shigella flexneri; Shigella sonnei

期刊名称：JOURNAL OF MEDICAL MICROBIOLOGY （ 影响因子：2.472； 五年影响因子：2.836 ）

ISSN： 0022-2615

年卷期： 2018 年 67 卷 3 期

页码：

收录情况： SCI

摘要： Purpose. Shigellosis is one of the most important food-borne and water-borne diseases worldwide. Although antibiotics are considered as efficient agents for shigellosis treatment, improper use of these has led to the emergence of antibiotic-resistant Shigella spp. Therefore, finding a new strategy as alternative treatment seems necessary. Methodology. Different samples from a wastewater treatment plant were used to isolate Shigella spp. specific phages. Physiological properties were determined, and genomic analysis was also carried out. Results. A virulent Siphoviridae bacteriophage, vB_SsoS-ISF002, was isolated from urban wastewater in Iran and showed infectivity to different isolates of both Shigella sonnei and Shigella flexneri. vB_SsoS-ISF002 was stable at different pH values and temperatures. It had a short latent period (15 min), a large burst size (76 +/- 9 p.f.u. cell(-1)) and appropriate lytic activity especially at high MOI. Its genome (dsDNA) was 50 564 bp with 45.53% GC content and 76 predicted open reading frames. According to comparative genomic analysis and phylogenic tree construction, vB_SsoS-ISF002 was considered as a member of the T1virus genus. Conclusion. These results indicated that vB_SsoS-ISF002 is a novel virulent T1virus phage and may have potential as an alternative treatment for shigellosis.

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