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BmCalpains are involved in autophagy and apoptosis during metamorphosis and after starvation in Bombyx mori

文献类型： 外文期刊

作者： Yi, Hui-Yu 1 ; Yang, Wan-Ying 1 ; Wu, Wen-Mei 1 ; Li, Xing-Xia 1 ; Deng, Xiao-Juan 1 ; Li, Qing-Rong 3 ; Cao, Yang 1 ; Zh 1 ;

作者机构： 1.South China Agr Univ, Coll Anim Sci, Guangdong Prov Key Lab Agroanim Genom & Mol Breed, Guangdong Prov Sericulture & Mulberry Engn Res Ct, Guangzhou 510642, Guangdong, Peoples R China

2.Jinan Univ, Coll Life Sci & Technol, Guangzhou 510632, Guangdong, Peoples R China

3.Guangdong Acad Agr Sci, Sericultural & Agri Food Res Inst, Guangzhou, Guangdong, Peoples R China

关键词： apoptosis; autophagy; BmATG6; Bombyx mori; calpain; starvation

期刊名称：INSECT SCIENCE （ 影响因子：3.262； 五年影响因子：3.206 ）

ISSN： 1672-9609

年卷期： 2018 年 25 卷 3 期

页码：

收录情况： SCI

摘要： Apoptosis and autophagy play crucial roles during Bombyx mori metamorphosis and in response to various adverse conditions, including starvation. Recently, calpain, one of the major intracellular proteases, has been reported to be involved in apoptosis and autophagy in mammals. BmATG5 and BmATG6 have been identified to mediate apoptosis following autophagy induced by 20-hydroxyecdysone and starvation in B. mori. However, B. mori calpains and their functions remain unclear. In this study, phylogenetic analysis of calpains from B. mori, Drosophila melanogaster and Homo sapiens were performed and the results showed distinct close relationships of BmCalpain-A/B with DmCalpain-A/B, BmCalpain-C with DmCalpain-C, and BmCalpain-7 with HsCalpain-7. Then, the expression profiles of BmCalpains were analyzed by quantitative real-time polymerase chain reaction, and results showed that expression of BmCalpain-A/B, BmCalpain-C and BmCalpain-7 was significantly increased during B. mori metamorphosis and induced in the fat body and midgut of starved larvae, which is consistent with the expression profiles of BmAtg5, BmAtg6 and BmCaspase-1. Moreover, the apoptosis-associated cleavage of BmATG6 in Bm-12 cells was significantly enhanced when BmCalpain-A/B and BmCalpain-7 were induced by starvation, and was partially inhibited by the inhibitor of either calpain or caspase, but completely inhibited when both types of inhibitors were applied together. Our results indicated that BmCalpains, including BmCalpain-A/B, -C and -7, may be involved in autophagy and apoptosis during B. mori metamorphosis and after starvation, and may also contribute to the apoptosis-associated cleavage of BmATG6.

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