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Steroid hormone 20-hydroxyecdysone induces the transcription and complex assembly of V-ATPases to facilitate autophagy in Bombyx mori

文献类型： 外文期刊

作者： Dai, Yichen 1 ; Li, Kang 2 ; Wu, Wenmei 1 ; Wu, Kunzhong 1 ; Yi, Huiyu 1 ; Li, Wangyu 1 ; Xiao, Yang 3 ; Zhong, Yangjin 1 ;

作者机构： 1.South China Agr Univ, Coll Anim Sci, Guangdong Prov Key Lab Agroanim Genom & Mol Breed, Guangdong Prov Sericulture & Mulberry Engn Res Ct, Guangzhou, Peoples R China

2.South China Normal Univ, Sch Life Sci, Inst Insect Sci & Technol, Guangdong Prov Key Lab Insect Dev Biol & Appl Tec, Guangzhou 510631, Peoples R China

3.Guangdong Acad Agr Sci, Sericultural & Agrifood Res Inst, Guangzhou 510610, Peoples R China

4.Southwest Univ, Biol Sci Res Ctr, Chongqing Engn & Technol Res Ctr Novel Silk Mat, Chongqing 400716, Peoples R China

关键词： 20-Hydroxyecdysone; Starvation; BmV-ATPases; BmTFEB; BmmTORCI; Autophagic flux

期刊名称：INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY （ 影响因子：4.714； 五年影响因子：4.953 ）

ISSN： 0965-1748

年卷期： 2020 年 116 卷

页码：

收录情况： SCI

摘要： Vacuolar-type H + -adenosine triphosphatases (V-ATPases) are indispensable for lysosome acidification and participate in autophagic processes. The steroid hormone 20-hydroxyecdysone (20E) predominantly induces autophagy and regulates insect larval molting and metamorphosis; however, the specific mechanism of lysosome acidification regulation by 20E remains unclear. Here, we showed that the developmental profiles of Bombyx V-ATPases were in accordance with autophagy occurrence and lysosome acidification in the fat body during larval pupal metamorphosis. BmV-ATPase-A and BmV-ATPase-B were required for lysosome acidification and autophagic flux. Both 20E treatment and starvation were able to induce lysosome acidification. Furthermore, BmV-ATPase transcription was induced by 20E treatment and reduced by RNAi targeting the 20E receptor BmUsp. On the one hand, 20E upregulated the transcription of BmV-ATPases through inducing Bombyx transcription factor EB (TFEB) and its nuclear translocation; on the other hand, 20E inhibited mTOR signaling to induce the transcription and assembly of BmV-ATPase subunits. Overall, 20E induces lysosome acidification by upregulating the transcription and assembly of V-ATPase subunits via activating BmTFEB and cooperating with nutrient signaling. These findings improve our understanding of the regulatory mechanisms underlying lysosome acidification and autophagic flux in Bombyx mori.

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