Steroid hormone 20-hydroxyecdysone induces the transcription and complex assembly of V-ATPases to facilitate autophagy in Bombyx mori
文献类型： 外文期刊
作者： Dai, Yichen 1 ; Li, Kang 2 ; Wu, Wenmei 1 ; Wu, Kunzhong 1 ; Yi, Huiyu 1 ; Li, Wangyu 1 ; Xiao, Yang 3 ; Zhong, Yangjin 1 ;
作者机构： 1.South China Agr Univ, Coll Anim Sci, Guangdong Prov Key Lab Agroanim Genom & Mol Breed, Guangdong Prov Sericulture & Mulberry Engn Res Ct, Guangzhou, Peoples R China
2.South China Normal Univ, Sch Life Sci, Inst Insect Sci & Technol, Guangdong Prov Key Lab Insect Dev Biol & Appl Tec, Guangzhou 510631, Peoples R China
3.Guangdong Acad Agr Sci, Sericultural & Agrifood Res Inst, Guangzhou 510610, Peoples R China
4.Southwest Univ, Biol Sci Res Ctr, Chongqing Engn & Technol Res Ctr Novel Silk Mat, Chongqing 400716, Peoples R China
关键词： 20-Hydroxyecdysone; Starvation; BmV-ATPases; BmTFEB; BmmTORCI; Autophagic flux
期刊名称：INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY （ 影响因子：4.714； 五年影响因子：4.953 ）
ISSN： 0965-1748
年卷期： 2020 年 116 卷
页码：
收录情况： SCI
摘要： Vacuolar-type H + -adenosine triphosphatases (V-ATPases) are indispensable for lysosome acidification and participate in autophagic processes. The steroid hormone 20-hydroxyecdysone (20E) predominantly induces autophagy and regulates insect larval molting and metamorphosis; however, the specific mechanism of lysosome acidification regulation by 20E remains unclear. Here, we showed that the developmental profiles of Bombyx V-ATPases were in accordance with autophagy occurrence and lysosome acidification in the fat body during larval pupal metamorphosis. BmV-ATPase-A and BmV-ATPase-B were required for lysosome acidification and autophagic flux. Both 20E treatment and starvation were able to induce lysosome acidification. Furthermore, BmV-ATPase transcription was induced by 20E treatment and reduced by RNAi targeting the 20E receptor BmUsp. On the one hand, 20E upregulated the transcription of BmV-ATPases through inducing Bombyx transcription factor EB (TFEB) and its nuclear translocation; on the other hand, 20E inhibited mTOR signaling to induce the transcription and assembly of BmV-ATPase subunits. Overall, 20E induces lysosome acidification by upregulating the transcription and assembly of V-ATPase subunits via activating BmTFEB and cooperating with nutrient signaling. These findings improve our understanding of the regulatory mechanisms underlying lysosome acidification and autophagic flux in Bombyx mori.
- 相关文献
作者其他论文 更多>>
-
Mulberry leaf extract reduces damage by regulating the oxidative response, immune response and intestinal flora of largemouth bass (Micropterus salmoides) under chronic heat stress
作者：Fu, Bing;Zhou, Donglai;Qiu, Xiaotong;Zheng, Jieqiu;Yang, Qiong;Xiao, Yang;Liao, Sentai;Li, Qingrong;Xing, Dongxu;Qiu, Xiaotong;Zheng, Jieqiu
关键词：mulberry leaf extract; largemouth bass; heat stress; intestinal flora; liver injury; immune ability
-
Hsp70-mediated manipulation of antimicrobial peptide-related genes promotes BmNPV proliferation in Bombyx mori at relatively high temperatures
作者：Zhang, Jie;Hu, Yingying;Feng, Jiawei;He, Na;Li, Xiaofeng;Liyi, Qingge;Yi, Huiyu;Yang, Wanying;Zhang, Jie;Yang, Qiong
关键词：Bombyx mori; BmNPV; Innate immunity; Host-pathogen interaction; Heat shock protein (Hsp); Antimicrobial peptide (AMP)
-
Baculovirus protein kinase 1 activates AMPK-protein phosphatase 5 axis to hijack transcription factor EB for self-proliferation
作者：Pan, Jiasheng;Li, Rongsong;Chen, Yin;Memon, Fareed Uddin;Wu, Kun;Hu, Jianghao;Xie, Xiaole;Deng, Jianhao;Xu, Rongting;Tian, Ling;Pan, Jiasheng;Li, Rongsong;Chen, Yin;Memon, Fareed Uddin;Wu, Kun;Hu, Jianghao;Xie, Xiaole;Deng, Jianhao;Xu, Rongting;Tian, Ling;Li, Xiaodong;Li, Kang;Li, Kang;Li, Kang;Zhang, Chuanxi
关键词：BmNPV protein kinase 1; B. mori serine/threonine protein phosphatase 5; BmTFEB; Dephosphorylation; Proliferation
-
Rheological Behavior, Textural Properties, and Antioxidant Activity of Porphyra yezoensis Polysaccharide
作者：Ji, Chenyang;Long, Xiaoshan;Qi, Bo;Hu, Xiao;Ji, Chenyang;Hu, Xiao;Ji, Chenyang;Wang, Jingjie;Cao, Yang;Wang, Jingjie;Cao, Yang;Qi, Bo;Hu, Xiao
关键词：
Porphyra yezoensis polysaccharide; rheological properties; gelation mechanism; calcium ions; antioxidant activities -
Lipid metabolites affected by deficient autophagy antagonize the occurrence of autophagy through AMPK signaling in insects
作者：Tian, Ling;Zhong, Qien;Yang, Yubei;Tian, Ling;Zhong, Qien;Yang, Yubei;Tian, Ling;Zhong, Qien;Yang, Yubei;Wu, Wenmei;Xiao, Yang;Li, Sheng;Li, Kang;Li, Kang
关键词：Autophagy;
Bombyx mori ;Drosophila melanogaster ; Lipid degradation; Metabolites -
Efficiency of microbial fermentation on microbial shifts, enzymatic activity, and transcriptions in black soldier fly larvae during the sugarcane waste conversion
作者：Memon, Fareed Uddin;Ahmad, Sheraz;Cui, Ying;Feng, Xingbao;Zeng, Peng;Huang, Zhijun;Tian, Ling;Memon, Fareed Uddin;Ahmad, Sheraz;Cui, Ying;Feng, Xingbao;Zeng, Peng;Huang, Zhijun;Tian, Ling;Li, Chiho;Nabi, Farhan;Tettamanti, Gianluca;Tettamanti, Gianluca;Chen, Ting
关键词：Microbial fermentation; Sugarcane waste; Black soldier fly; Microbial shifts; Lignocellulose degradation; Transcriptional profiles
-
Transcriptomics and Metabolomics Combined to Analyze the Response Mechanism of Silkworm Eggs to High-Temperature Stress
作者：Xiao, Yang;Li, Qingrong;Sun, Zhenbo;Fu, Bing;Yang, Qiong;Zhang, Weilong;Xing, Dongxu;Jiang, Mangui;Huang, Xuhua
关键词：transcriptomics; metabonomics; silkworm egg; instant acid-impregnated eggs; high temperature stress
