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Immunogenicity evaluation of MS2 phage-mediated chimeric nanoparticle displaying an immunodominant B cell epitope of foot-and-mouth disease virus

文献类型： 外文期刊

作者： Wang, Guoqiang 1 ; Liu, Yunchao 2 ; Feng, Hua 2 ; Chen, Yumei 3 ; Yang, Suzhen 2 ; Wei, Qiang 2 ; Wang, Juan 4 ; Li, Dong 1 ;

作者机构： 1.Henan Agr Univ, Coll Anim Sci & Vet Med, Zhengzhou, Henan, Peoples R China

2.Henan Acad Agr Sci, Henan Prov Key Lab Anim Immunol, Zhengzhou, Henan, Peoples R China

3.Zhengzhou Univ, Sch Life Sci, Zhengzhou, Henan, Peoples R China

4.Henan Zhongze Biol Engn Co Ltd, Zhengzhou, Henan, Peoples R China

5.Yangzhou Univ, Jiangsu Coinnovat Ctr Prevent & Control Important, Yangzhou, Jiangsu, Peoples R China

关键词： Foot-and-mouth disease virus; Chimeric nanoparticles; MS2 bacteriophage; G-H loop

期刊名称：PEERJ （ 影响因子：2.984； 五年影响因子：3.369 ）

ISSN： 2167-8359

年卷期： 2018 年 6 卷

页码：

收录情况： SCI

摘要： Foot-and-mouth disease (FMD) is a highly contagious disease of cloven-hoofed animals that has caused tremendous economic losses worldwide. In this study, we designed a chimeric nanoparticles (GNPs) vaccine that displays the predominant epitope of the serotype 0 foot-and-mouth disease virus (FMDV) VP]. 131-160 on the surface of MS2 phage. The recombinant protein was expressed in Escherichia Coli and can self -assemble into GNPs with diameter at 25-30 nm in vitro. A tandem repeat peptide epitopes (TRE) was prepared as control. Mice were immunized with GNPs, TRE and commercialized synthetic peptide vaccines (PepVac), respectively. The ELISA results showed that CNPs stimulated a little higher specific antibody levels to PepVac, but was significantly higher than the TRE groups. Moreover, the results from specific IFN-gamma responses and lymphocyte profileration test indicated that CNP immunized miceresponses and lymphocyte proliferation test that GNP immunized mice exhibited significantly enhanced cellular immune response compared to TRE. These results suggested that the GNPs constructed in current study could be a potential alternative vaccine in future FMDV control.

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