Triple helix conformation of beta-D-glucan from Ganoderma lucidum and effect of molecular weight on its immunostimulatory activity
文献类型： 外文期刊
作者： Liu, Yanfang 1 ; Tang, Qingjiu 2 ; Zhang, Jingsong 2 ; Xia, Yongmei 1 ; Yang, Yan 2 ; Wu, Di 2 ; Fan, Hua 4 ; Cui, Steve 1 ;
作者机构： 1.Jiangnan Univ, Sch Chem & Mat Engn, State Key Lab Food Sci & Technol, Wuxi 214122, Peoples R China
2.Shanghai Acad Agr Sci, Inst Edible Fungi, Shanghai 201403, Peoples R China
3.Agr & Agri Food Canada, Guelph Food Res Ctr, 93 Stone Rd W, Guelph, ON N1G 5C9, Canada
4.Charite Univ Med Berlin, Inst Lab Med Clin Chem & Pathobiochem, Campus Virchow Clin, Berlin, Germany
关键词： Ganoderma lucidum; beta-D-glucan; Triple-helix; Polysaccharide degradation; Macrophages; Inflammatory cytokines
期刊名称：INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES （ 影响因子：6.953； 五年影响因子：6.737 ）
ISSN： 0141-8130
年卷期： 2018 年 114 卷
页码：
收录情况： SCI
摘要： beta-D-glucan (GLP20) isolated from Ganoderma lucidum fruiting bodies was successfully fractionated into five fractions with different weight-average molecular weights (M-w) through ultrasonic irradiation. The M-w, radius of gyration (R-g), hydrodynamic radius (R-h) and intrinsic viscosity ([eta]) of these fractions in phosphate buffered saline (PBS) aqueous solution were determined using HPSEC-MALLS-RI-VS system. The results indicated that beta-D-glucan displayed rigid chain conformations with rho values lager than 2.06 for the five fractions. By applying the polymer solution theory, the exponent (nu and alpha) values of R-g = kM(W)(nu)) and [eta] = kM(W)(alpha) were calculated as 0.72 and 1.05, respectively, which confirmed the rigid chain conformation of glucans with M-w from 2.9 x 10(5) to 2.42 x 10(6) g.mol(-1). According to the known theory for chains, the molar mass per unit contour length M-L, persistence length q and contour length h per main-chain glucose residue were estimated to be 2150 nm(-1), 128 nm and 0.30 nm, respectively, which indicated that the glucan existed as triple-helical chains in PBS aqueous solution. Bioactivity study of glucan fractions on lymphocytes and THP-1 macrophages demonstrated that all these fractions could stimulate lymphocyte proliferation, promote macrophages to form pseudopodia, and enhance the levels of inflammatory cytokines IL-6 and TNF-alpha. Meanwhile, the glucan fractions with M-w higher than 1.82 x 10(6) g.mol(-1) exhibited better activity on enhancing the release of inflammatory cytokines. (C) 2018 Elsevier B.V. All rights reserved.
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