文献类型： 外文期刊
作者： Liu, Zhao-Xin 1 ; Han, Zhenggang 1 ; Yu, Xiao-Li 1 ; Wen, Guoyuan 3 ; Zeng, Chi 1 ;
作者机构： 1.Wuhan Polytech Univ, Sch Biol & Pharmaceut Engn, Hubei Prov Engn Res Ctr Hlth Food, Wuhan 430023, Hubei, Peoples R China
2.Wuhan Univ, Coll Life Sci, Wuhan 430072, Hubei, Peoples R China
3.Hubei Acad Agr Sci, Inst Anim Husb & Vet, Key Lab Prevent & Control Agents Anim Bacteriosis, Minist Agr, Wuhan 430064, Hubei, Peoples R China
关键词： polymyxin resistance; colistin resistance; MCR-1
期刊名称：CRYSTALS （ 影响因子：2.589； 五年影响因子：2.615 ）
ISSN： 2073-4352
年卷期： 2018 年 8 卷 4 期
页码：
收录情况： SCI
摘要： The polymyxin colistin is known as a "last resort" antibacterial drug toward pandrug-resistant enterobacteria. The recently discovered plasmid-encoded mcr-1 gene spreads rapidly across pathogenic strains and confers resistance to colistin, which has emerged as a global threat. The mcr-1 gene encodes a phosphoethanolamine transferase (MCR-1) that catalyzes the transference of phosphoethanolamine to lipid A moiety of lipopolysaccharide, resulting in resistance to colistin. Development of effective MCR-1 inhibitors is crucial for combating MCR-1-mediated colistin resistance. In this study, MCR-1 catalytic domain (namely cMCR-1) was expressed and co-crystallized together with D-xylose. X-ray crystallographic study at a resolution of 1.8 angstrom found that cMCR-1-d-xylose co-crystals fell under space group P2(1)2(1)2(1), with unit-cell parameters a = 51.6 angstrom, b = 73.1 angstrom, c = 82.2 angstrom, alpha = 90 degrees, beta = 90 degrees, gamma = 90 degrees. The asymmetric unit contained a single cMCR-1 molecule complexed with d-xylose and had a solvent content of 29.13%. The structural model of cMCR-1-d-xylose complex showed that a D-xylose molecule bound in the putative lipid A-binding pocket of cMCR-1, which might provide a clue for MCR-1 inhibitor development.
- 相关文献
作者其他论文 更多>>
-
Research note: Prevalence and genetic characteristics of pathogenic E. coli isolates from domestic pigeons in central China
作者：Wang, Juan;Lu, Qin;Yao, Lun;Zhang, Wenting;Hu, Qiao;Guo, Yunqing;Wen, Guoyuan;Shao, Huabin;Luo, Qingping;Zhang, Tengfei;Wen, Guoyuan;Luo, Qingping
关键词：Pathogenic E. coli; Pigeons; Serotypes; Antibiotic resistance
-
Construction and bacteriostatic effect analyses of a recombinant thermostable Newcastle disease virus expressing cecropin AD
作者：Yao, Lun;Ren, Xiangfei;Yang, Junjie;Zeng, Zhe;Shang, Yu;Feng, Helong;Jin, Mengyun;Xiao, Qianni;Shao, Huabin;Luo, Qingping;Wen, Guoyuan;Ren, Xiangfei;Yang, Junjie;Hu, Sishun;Yao, Lun;Ren, Xiangfei;Yang, Junjie;Zeng, Zhe;Shang, Yu;Feng, Helong;Jin, Mengyun;Xiao, Qianni;Shao, Huabin;Luo, Qingping;Wen, Guoyuan;Zhou, Ping;Zeng, Chi;Luo, Qingping
关键词：Newcastle disease virus; Cecropin AD; Staphylococcus aureus; Wound healing
-
Addressing unexpected bacterial RNA safety concerns of E. coli produced influenza NP through CpG loaded mutant
作者：Chen, Cen;Li, Mengling;Guo, Aili;Guo, Pengju;Zhang, Wanpo;Gu, Changqin;Zhou, Hongbo;Tao, Pan;Chen, Cen;Li, Mengling;Guo, Aili;Guo, Pengju;Zhou, Hongbo;Tao, Pan;Wen, Guoyuan
关键词：
-
Genetic and pathogenic characteristics of a novel recombinant GI-19 infectious bronchitis virus strain isolated from northeastern China
作者：Zeng, Zhe;Yao, Lun;Feng, Helong;Wang, Zichen;Jiang, Liren;Wang, Haojie;Shang, Yu;Wang, Hongcai;Shao, Huabin;Wen, Guoyuan;Luo, Qingping;Zeng, Zhe;Yao, Lun;Feng, Helong;Wang, Zichen;Jiang, Liren;Wang, Haojie;Shang, Yu;Wang, Hongcai;Shao, Huabin;Wen, Guoyuan;Luo, Qingping;Zeng, Zhe;Yao, Lun;Feng, Helong;Wang, Zichen;Jiang, Liren;Wang, Haojie;Shang, Yu;Wang, Hongcai;Shao, Huabin;Wen, Guoyuan;Luo, Qingping;Wen, Guoyuan;Luo, Qingping;Feng, Helong;Zhou, Chengli
关键词：Infectious bronchitis virus; GI-19; Evolutionary; Recombination; Pathogenicity; Antigenic variation
-
The impacts of animal agriculture on One Health-Bacterial zoonosis, antimicrobial resistance, and beyond
作者：Zhang, Tengfei;Zhang, Wenting;Shang, Yu;Luo, Qingping;Wen, Guoyuan;Nickerson, Rhea;Cheng, Zhenyu;Peng, Xitian;Zhou, Youxiang;Peng, Xitian;Zhou, Youxiang;Peng, Xitian;Zhou, Youxiang;Luo, Qingping
关键词：Zoonosis; Antimicrobial resistance
-
BacScan: a novel genome-wide strategy for uncovering broadly immunogenic proteins in bacteria
作者：Dong, Junhua;Zhang, Qian;Yang, Jinyue;Zhao, Yacan;Miao, Zhuangxia;Pei, Siyang;Jing, Changwei;Zhang, Anding;Tao, Pan;Dong, Junhua;Zhang, Qian;Yang, Jinyue;Zhao, Yacan;Miao, Zhuangxia;Pei, Siyang;Jing, Changwei;Zhang, Anding;Tao, Pan;Dong, Junhua;Zhang, Qian;Yang, Jinyue;Zhao, Yacan;Miao, Zhuangxia;Pei, Siyang;Jing, Changwei;Zhang, Anding;Tao, Pan;Qin, Huan;Wen, Guoyuan
关键词：bacterial subunit vaccine; vaccine target; immunogenic protein; protective antigen; genome-wide screening; streptococcus suis
-
Spray vaccination with a Newcastle disease virus (NDV)-vectored infectious laryngotracheitis (ILT) vaccine protects commercial chickens from ILT in the presence of maternally-derived antibodies
作者：Zeng, Zhe;Wang, Zichen;Wang, Xin;Yao, Lun;Shang, Yu;Feng, Helong;Wang, Hongcai;Shao, Huabin;Luo, Qingping;Wen, Guoyuan;Zeng, Zhe;Wang, Zichen;Wang, Xin;Yao, Lun;Shang, Yu;Feng, Helong;Wang, Hongcai;Shao, Huabin;Luo, Qingping;Wen, Guoyuan;Zeng, Zhe;Wang, Zichen;Wang, Xin;Yao, Lun;Shang, Yu;Feng, Helong;Wang, Hongcai;Shao, Huabin;Luo, Qingping;Wen, Guoyuan;Luo, Qingping;Wen, Guoyuan
关键词：ILTV; NDV; vector vaccine; spray vaccination; maternally derived antibodies; protection
