Physiological, proteomic, and gene expression analysis of turbot (Scophthalmus maximus) in response to cold acclimation
文献类型： 外文期刊
作者： Nie, Xiaobao 1 ; Lei, Jilin 2 ; Chen, Shixi 1 ; Zhang, Yuting 1 ; Zhang, Changfeng 3 ; Hong, Wanshu 1 ;
作者机构： 1.Xiamen Univ, Coll Ocean & Earth Sci, Xiamen 361102, Peoples R China
2.Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Qingdao 266071, Peoples R China
3.Shandong Key Lab Storage & Transportat Technol Ag, Jinan 250103, Shandong, Peoples R China
4.Natl Engn Res Ctr Agr Prod Logist, Jinan 250103, Shandong, Peoples R China
关键词： Cold acclimation; Fish preservation; Proteome analysis; Turbot; Physiological response; qRT-PCR
期刊名称：AQUACULTURE （ 影响因子：4.242； 五年影响因子：4.723 ）
ISSN： 0044-8486
年卷期： 2018 年 495 卷
页码：
收录情况： SCI
摘要： Cold acclimation has a tremendous impact on the survivability and the freshness of turbot when presented to consumers for sale. In the present study, to better understand the response of turbot to cold acclimation, physiological parameters determination, two-dimensional-based proteomic analysis and the expression of target genes by quantitative real-time PCR were analyzed. The results of physiological parameters indicated that the levels of glucose, ATP and cortisol, and the activities of lysozyme, catalase (CAT) and superoxide dismutase (SOD) were elevated. Moreover, the liver damage may be determined due to the increase of glutamic-oxalacetic transaminase (GOT) and glutamic-pyruvic transaminase (GPT) in serum. Furthermore, a total of sixteen differentially accumulated proteins were identified, which are mainly involved in carbohydrate and energy metabolism. Based on these data, we concluded that turbot accelerate the glycogen hydrolysis in liver and muscle to increase the glucose level in serum and promote the energy metabolism, enhance innate immune and heighten the antioxidant capacity to respond to cold acclimation. Meanwhile, the cold acclimation can also cause adverse effect to the innate immune system and liver of turbot. These results are helpful in understanding the response of turbot to cold acclimation and in providing the theoretical base for applying fish cold acclimation in transport.
- 相关文献
作者其他论文 更多>>
-
Effects of Glyphosate-Resistant Genetically Modified Soybean on Blood Biochemical Indexes, Hepatopancreatic Antioxidant Capacity and Tissue Morphology of Cyprinus carpio
作者：Zhang, Jiaguo;Yu, Taotao;Huang, Xiaoxia;Ma, Qianqian;Zhang, Changfeng;Zhang, Jiaguo;Ma, Qianqian;Zhang, Changfeng;Yu, Taotao;Huang, Xiaoxia;Zhou, Qunlan
关键词：Glyphosate-resistant genetically modified soybean; Cyprinus carpio; biochemical indexes; antioxidant capacity; tissue morphology
-
Transcriptome analysis reveals the mechanism of alkalinity exposure on spleen oxidative stress, inflammation and immune function of Luciobarbus capito
作者：Shang, Xinchi;Geng, Longwu;Yang, Jian;Zhang, Yuting;Xu, Wei;Shang, Xinchi;Geng, Longwu;Yang, Jian;Zhang, Yuting;Xu, Wei
关键词：Transcriptome; Alkalinity; Oxidative stress; Inflammation; Immune
-
Effect of Pectin Coating Infused with Gallic Acid on the Quality and Shelf Life of Japanese Sea Bass (Lateolabrax japonicas) Fillets
作者：Nie, Xiaobao;Ren, Xinrong;Jiang, Qiannan;Li, Songlin;Jiang, Changxing;Gao, Zhe;Liu, Bin;Liu, Xinfu;He, Fangyun
关键词：Gallic acid; Japanese sea bass; Pectin; Quality; Refrigeration
-
Molecular function of gonadotrophins and their receptors in the ovarian development of turbot (Scophthalmus maximus)
作者：Jia, Yudong;Lei, Jilin;Jia, Yudong
关键词：Follicle-stimulating hormone (FSH); Luteinizing hormone (LH); FSH receptor; LH receptor; Reproduction; Turbot (Scophthalmus maximus)
-
The complete mitochondrial genome sequence of the giant clam Tridacna derasa (Tridacnidae: Tridacna)
作者：Ma, Haitao;Zhang, Yuehuan;Chen, Shixi;Shi, Wei;Yu, Ziniu;Lin, Lin
关键词：Tridacna derasa; mitochondrial genome; phylogenetic relationship
-
Effect of a Sodium Alginate Coating Infused with Tea Polyphenols on the Quality of Fresh Japanese Sea Bass (Lateolabrax japonicas) Fillets
作者：Nie, Xiaobao;Hong, Wanshu;Nie, Xiaobao;Wang, Lihong;Wang, Qi;Huang, Baosheng;Zhang, Changfeng;Lei, Jilin;Wang, Lihong;Wang, Qi;Huang, Baosheng;Zhang, Changfeng
关键词：fillet quality; Lateolabrax japonicas; refrigerate storage; sodium alginate; tea polyphenol
-
Complete mitochondria! genome of Toxabramis houdemeri (Cypriniformes; Cyprinidae) and its phylogenetic analysis
作者：Wang, Pengfei;Chen, Shixi;Ou, Youjun;Wen, Jiufu;Li, Jia'er
关键词：Cultrinae; Cyprinidae; mitochondrial genome; Toxabramis houdemeri
