High and efficient isomaltulose production using an engineered Yarrowia lipolytica strain
文献类型： 外文期刊
作者： Zhang, Peng 1 ; Wang, Zhi-Peng 2 ; Sheng, Jun 2 ; Zheng, Yuan 2 ; Ji, Xiao-Feng 2 ; Zhou, Hai-Xiang 1 ; Liu, Xiao-Yan; 1 ;
作者机构： 1.Chinese Acad Agr Sci, Tobacco Res Inst, Qingdao 266101, Shandong, Peoples R China
2.Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Minist Agr & Rural Affairs, Key Lab Sustainable Dev Polar Fishery, Qingdao 266071, Shandong, Peoples R China
3.Huaiyin Normal Univ, Jiangsu Key Lab Biomass Based Energy & Enzyme Tec, Huaian 223300, Jiangsu, Peoples R China
4.Ocean Univ China, Coll Marine Life Sci, Qingdao 266003, Shandong, Peoples R C
关键词： Isomaltulose; Sucrose isomerase; Yarrowia lipolytica; Invertase
期刊名称：BIORESOURCE TECHNOLOGY （ 影响因子：9.642； 五年影响因子：9.237 ）
ISSN： 0960-8524
年卷期： 2018 年 265 卷
页码：
收录情况： SCI
摘要： Isomaltulose is an ideal functional sweetener and has been approved as a safe sucrose substitute. It is produced mainly through sucrose isomerization catalyzed by sucrose isomerase. Here, to produce food-grade isomaltulose and improve its yield, the sucrose isomerase gene from Pantoea dispersa UQ68J was overexpressed in the nonpathogenic yeast Yarrowia lipolytica. When the engineered strain, S47, was fermented on 600 g/L sucrose in a 10L bioreactor, a maximum isomaltulose concentration of 572.1 g/L was achieved. Sucrose isomerase activity was 7.43 U/mL, and yield reached 0.96 g/g. Moreover, monosaccharide byproducts were simultaneously transformed into intracellular lipids, thus reducing the production of undesirable compounds and resulting in high isomaltulose purity (97.8%) in the final broth. In summary, the bioprocess employed in this study provides an efficient alternative strategy for isomaltulose production.
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