Coupling oligonucleotides possessing a poly-cytosine tag with magnetic ionic liquids for sequence-specific DNA analysis
文献类型： 外文期刊
作者： Peng, Xitian 1 ; Clark, Kevin D. 2 ; Ding, Xiong 2 ; Zhu, Chenghui 2 ; Varona, Marcelino 2 ; Emaus, Miranda N. 2 ; An, 1 ;
作者机构： 1.Hubei Acad Agr Sci, Inst Agr Qual Stand & Testing Technol Res, Wuhan 430064, Hubei, Peoples R China
2.Iowa State Univ, Dept Chem, Ames, IA 50011 USA
期刊名称：CHEMICAL COMMUNICATIONS （ 影响因子：6.222； 五年影响因子：6.008 ）
ISSN： 1359-7345
年卷期： 2018 年 54 卷 73 期
页码：
收录情况： SCI
摘要： Oligonucleotide probes were designed with a poly-cytosine region that facilitates stable anchoring to a magnetic ionic liquid support. By tethering a recognition sequence to the poly-C tag, the resulting diblock oligonucleotides distinguished single-nucleotide variants and captured DNA targets from interfering genomic DNA and cell lysate for qPCR amplification.
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