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Endophytic Pseudomonas induces metabolic flux changes that enhance medicinal sesquiterpenoid accumulation in Atractylodes lancea

文献类型： 外文期刊

作者： Zhou, Jia Yu 1 ; Sun, Kai 1 ; Chen, Fei 1 ; Yuan, Jie 1 ; Li, Xia 3 ; Dai, Chuan-Chao 1 ;

作者机构： 1.Nanjing Normal Univ, Coll Life Sci, Jiangsu Engn & Technol Res Ctr Ind Microbial Reso, Jiangsu Key Lab Microbes & Funct Genom, Nanjing 210023, Jiangsu, Peoples R China

2.Jiangsu Prov & Chinese Acad Sci, Inst Bot, Nanjing 210014, Jiangsu, Peoples R China

3.Jiangsu Acad Agr Sci, Jiangsu High Qual Rice R&D Ctr, Inst Food Crops, Nanjing 210014, Jiangsu

关键词： Atractylodes lancea; Bacterial endophyte; Conversion; Metabolic flux change; Medicinal terpenoid; Microbial elicitor

期刊名称：PLANT PHYSIOLOGY AND BIOCHEMISTRY （ 影响因子：4.27； 五年影响因子：4.816 ）

ISSN： 0981-9428

年卷期： 2018 年 130 卷

页码：

收录情况： SCI

摘要： The bacterial endophyte Pseudomonas fluorescens ALEB7B significantly enhances photosynthate accumulations in Atractylodes lancea. These carbohydrates are preferentially used by the host plant to synthesize secondary metabolites, rather than to increase plant biomass accumulation. Mechanisms underlying the allocation of endophyte-increased carbohydrate in different plant metabolic processes are largely unknown. We have studied how P. fluorescens ALEB7B enhances photosynthate accumulation and how bacterial elicitors regulate metabolic flux and increase medicinal sesquiterpenoid formation in A. lancea using the sterile tissue culture plantlets. P. fluorescens ALEB7B enhances plant photosynthate accumulation by synthesizing and secreting indole-3-acetic acid, which has been demonstrated using high-performance liquid chromatography analysis. The increased endogenous indole-3-acetic acid promotes plant root development and then assimilation. Increased carbohydrates provide the material basis for the formations of terpenoid hydrocarbon scaffolds, which has been proved using gas chromatography analysis. Further, protein and polysaccharide elicitors secreted by P. fluorescens ALEB7B have been separated and purified from the bacterial fermentation broth, which have been applied to A. lancea plantlets. Both elicitors can stimulate the conversions of terpenoid hydrocarbon scaffolds to oxygenous sesquiterpenoids, the active medicinal ingredients in A. lancea, by triggering the oxidative burst in planta. Moreover, this study separates an ABC transporter substrate-binding protein from protein elicitors secreted by P. fluorescens ALEB7B with an AKTA Prime Plus Purifier System and firstly shows that this protein is essential to induce oxygenous sesquiterpenoid accumulation in A. lancea. This study provides new perspectives for mechanisms of medicinal oxygenous terpenoid synthesis, which has important reference values to the cultivation of medicinal plants that have terpenoids as their active ingredients, such as Artemisia annua and Taxus chinensis.

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