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The Redox-Sensing Regulator Rex Contributes to the Virulence and Oxidative Stress Response of Streptococcus suis Serotype 2

文献类型： 外文期刊

作者： Zhu, Haodan 1 ; Wang, Yong 1 ; Ni, Yanxiu 1 ; Zhou, Junming 1 ; Han, Lixiao 1 ; Yu, Zhengyu 1 ; Mao, Aihua 1 ; Wang, Dand 1 ;

作者机构： 1.Jiangsu Acad Agr Sci, Inst Vet Med, Nanjing, Jiangsu, Peoples R China

2.Yangzhou Univ, Jiangsu Coinnovat Ctr Prevent & Control Important, Yangzhou, Jiangsu, Peoples R China

3.Nanjing Agr Univ, Coll Vet Med, Nanjing, Jiangsu, Peoples R China

4.State Key Lab Breeding Base, Key Lab Food Qual & Safety Jiangsu Prov, Nanjing, Jiangsu, Peoples R China

关键词： Streptococcus suis; redox-sensing regulator Rex; virulence; oxidative stress; electrophoretic mobility shift assays

期刊名称：FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY （ 影响因子：5.293； 五年影响因子：5.882 ）

ISSN： 2235-2988

年卷期： 2018 年 8 卷

页码：

收录情况： SCI

摘要： Streptococcus suis serotype 2 (SS2) is an important zoonotic pathogen responsible for septicemia and meningitis. The redox-sensing regulator Rex has been reported to play critical roles in the metabolism regulation, oxidative stress response, and virulence of various pathogens. In this study, we identified and characterized a Rex ortholog in the SS2 virulent strain SS2-1 that is involved in bacterial pathogenicity and stress environment susceptibility. Our data show that the Rex-knockout mutant strain Delta rex exhibited impaired growth in medium with hydrogen peroxide or a low pH compared with the wildtype strain SS2-1 and the complementary strain C Delta rex. In addition, Delta rex showed a decreased level of survival in whole blood and in RAW264.7 macrophages. Further analyses revealed that Rex deficiency significantly attenuated bacterial virulence in an animal model. A comparative proteome analysis found that the expression levels of several proteins involved in virulence and oxidative stress were significantly different in Delta rex compared with SS2-1. Electrophoretic mobility shift assays revealed that recombinant Rex specifically bound to the promoters of target genes in a manner that was modulated by NADH and NAD(+). Taken together, our data suggest that Rex plays critical roles in the virulence and oxidative stress response of SS2.

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