Effects of Various Treatments on Extraction of the Main Bioactive Components and Determination of Biological Activity of Extracts from the Caterpillar Medicinal Mushroom Cordyceps militaris (Ascomycetes)
文献类型： 外文期刊
作者： Yin, Chaomin 1 ; Fan, Xiuzhi 1 ; Fan, Zhe 1 ; Shi, Defang 1 ; Gao, Hong 1 ;
作者机构： 1.Hubei Acad Agr Sci, Inst Agroprod Proc & Nucl Agr Technol, 5 Nanhu Ave, Wuhan 430064, Hubei, Peoples R China; Natl Res & Dev Ctr Edible Fungi Proc, Wuhan, Hubei, Peoples R China
关键词： adenosine; antioxidant activities; cordycepin; Cordyceps militaris; medicinal mushrooms; orthogonal test; polysaccharides; xanthine oxidase inhibitory activity
期刊名称：INTERNATIONAL JOURNAL OF MEDICINAL MUSHROOMS （ 影响因子：1.921； 五年影响因子：1.879 ）
ISSN： 1521-9437
年卷期： 2018 年 20 卷 9 期
页码：
收录情况： SCI
摘要： In this study we optimized the extraction of cordycepin from Cordyceps militaris through the use of an orthogonal test based on single-factor experiments. The results showed that the hugest amount of cordycepin (1260.28 +/- 46.38 mu g/g) was achieved with an extraction time of 15 minutes, an extraction temperature of 100 degrees C, and a liquid-to-material ratio of 50 mL/g. The antioxidant activities and xanthine oxidase inhibitory activity of aqueous extracts, ethanol extracts, and polysaccharides from C. militaris were subsequently determined. The results showed that the various extracts and polysaccharides all had certain antioxidant activities. However, the polysaccharides had a strong ability to scavenge superoxide radicals and at 0.5-3.0 mg/mL had almost the same effect as vitamin C. In addition, the polysaccharides displayed concentration-dependent xanthine oxidase inhibitory activity, but the ethanol extract exhibited a negative relation at concentrations of 0.5-3.0 mg/mL, whereas the aqueous extracts had very low (< 10%) xanthine oxidase inhibitory activity. Our results indicate that C. militaris has potential use as part of well-balanced diets and in the pharmaceutical industry.
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