Establishment and characterization of a telomerase-immortalized porcine bronchial epithelial cell line
文献类型： 外文期刊
作者： Xie, Xing 1 ; Gan, Yuan 1 ; Pang, Maoda 2 ; Shao, Guoqing 1 ; Zhang, Lei 1 ; Liu, Beibei 1 ; Xu, Qi 3 ; Wang, Haiyan 1 ; Fen 1 ;
作者机构： 1.Jiangsu Acad Agr Sci, Inst Vet Med, Key Lab Vet Biol Engn & Technol, Minist Agr, 50 Zhongling St, Nanjing 210014, Jiangsu, Peoples R China
2.Jiangsu Acad Agr Sci, Inst Food Safety & Nutr, Key Lab Control Technol & Stand Agroprod Safety &, Key Lab Food Qual & Safety Jiangsu Prov,State Key, Nanjing, Jiangsu, Peoples R China
3.Yangzhou Univ, Coll Anim Sci & Technol, Yangzhou, Jiangsu, Peoples R China
4.Nanjing Agr Univ, Coll Vet Med, Nanjing, Jiangsu, Peoples R China
关键词： cell model; hTERT-PBECs; porcine bronchial epithelial cells; porcine respiratory disease
期刊名称：JOURNAL OF CELLULAR PHYSIOLOGY （ 影响因子：6.384； 五年影响因子：5.987 ）
ISSN： 0021-9541
年卷期： 2018 年 233 卷 12 期
页码：
收录情况： SCI
摘要： Primary porcine bronchial epithelial cells (PBECs) are an ideal model to study the molecular and pathogenic mechanisms of various porcine respiratory pathogens. However, the short lifespan of primary PBECs greatly limit their application. Here, we isolated and cultured primary PBECs and established immortalized PBECs by transfecting primary PBECs with the pEGFP-hTERT recombinant plasmid containing human telomerase reverse transcriptase (hTERT). Immortalized PBECs (hTERT-PBECs) retained the morphological and functional features of primary PBECs as indicated by cytokeratin 18 expression, telomerase activity assay, proliferation assays, karyotype analysis, and quantitative reverse-transcriptase polymerase chain reaction. Compared to primary PBECs, hTERT-PBECs had higher telomerase activity, extended replicative lifespan, and displayed enhanced proliferative activity. Moreover, this cell line is not transformed in vitro and does not exhibit a malignant phenotype in vivo, suggesting that it can be safely used in further studies. Besides, hTERT-PBECs were susceptible to swine influenza virus of H3N2 subtype and porcine circovirus type 2. In conclusion, the immortalized hTERT-PBECs represent a valuable in vitro model, which can be widely used in the study of porcine respiratory pathogenic infections.
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