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The protein-sparing effect of alpha-lipoic acid in juvenile grass carp, Ctenopharyngodon idellus: effects on lipolysis, fatty acid beta-oxidation and protein synthesis

文献类型： 外文期刊

作者： Shi, Xiao-chen 1 ; Jin, Ai 1 ; Sun, Jian 1 ; Tian, Jing-jing 2 ; Ji, Hong 1 ; Chen, Li-qiao 3 ; Du, Zhen-yu 3 ;

作者机构： 1.Northwest A&F Univ, Coll Anim Sci & Technol, Yangling 712100, Shaanxi, Peoples R China

2.Chinese Acad Fishery Sci, Minist Agr, Pearl River Fisheries Res Inst, Key Lab Trop & Subtrop Fishery Resource Applicat, Guangzhou 510380, Guangdong, Peoples R China

3.East China Normal Univ, Sch Life Sci, Shanghai 200241, Peoples R China

关键词： alpha-Lipoic acid; Protein-sparing effect; Lipolysis; Fatty acid beta-oxidation; Ctenopharyngodon idellus

期刊名称：BRITISH JOURNAL OF NUTRITION （ 影响因子：3.718； 五年影响因子：4.816 ）

ISSN： 0007-1145

年卷期： 2018 年 120 卷 9 期

页码：

收录情况： SCI

摘要： To investigate the protein-sparing effect of alpha-lipoic acid (LA), experimental fish (initial body weight: 18.99 (SD 1.82) g) were fed on a 0, 600 or 1200 mg/kg alpha-LA diet for 56 d, and hepatocytes were treated with 20 mu M compound C, the inhibitor of AMP kinase alpha (AMPK alpha), treated for 30 min before alpha-LA treatment for 24 h. LA significantly decreased lipid content of the whole body and other tissues (P < 0.05), and it also promoted protein deposition in vivo (P < 0.05). Further, dietary LA significantly decreased the TAG content of serum and increased the NEFA content of serum (P < 0.05); however, there were no significant differences among all groups in the hepatopancreas and muscle (P > 0.05). Consistent with results from the experiment in vitro, LA activated phosphorylation of AMPK alpha and notably increased the protein content of adipose TAG lipase in intraperitoneal fat, hepatopancreas and muscle in vivo (P < 0.05). Meanwhile, LA significantly up-regulated the mRNA expression of genes involved in fatty acid beta-oxidation in the same three areas, and LA also obviously down-regulated the mRNA expression of genes involved in amino acid catabolism in muscle (P < 0.05). Besides, it was observed that LA significantly activated the mammalian target of rapamycin (mTOR) pathway in muscle of experimental fish (P < 0.05). LA could promote lipolysis and fatty acid beta-oxidation via increasing energy supply from lipid catabolism, and then, it could economise on the protein from energy production to increase protein deposition in grass carp. Besides, LA might directly promote protein synthesis through activating the mTOR pathway.

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