Development of a method for the quantification of fish major allergen parvalbumin in food matrix via liquid chromatography-tandem mass spectrometry with multiple reaction monitoring
文献类型： 外文期刊
作者： Sun, Lirui 1 ; Li, Hong 1 ; Li, Zhenxing 1 ; Sun, Weihong 2 ; Wang, Jianhua 3 ; Wu, Haiyan 2 ; Ge, Minmin 3 ; Ahmed, Ishf 1 ;
作者机构： 1.Ocean Univ China, Coll Food Sci & Engn, 5 Yushan Rd, Qingdao 266003, Shandong, Peoples R China
2.Chinese Acad Fishery Sci, Minist Agr, Key Lab Testing & Evaluat Aquat Prod Safety & Qua, Yellow Sea Fisheries Res Inst, Qingdao 266071, Peoples R China
3.Shandong Entry Exit Inspect & Quarantine Bur, Tech Ctr Inspect & Quarantine, Qingdao 266002, Shandong, Peoples R China
关键词： Allergen; Parvalbumin; Liquid chromatography; Mass spectrometry; Quantification
期刊名称：FOOD CHEMISTRY （ 影响因子：7.514； 五年影响因子：7.516 ）
ISSN： 0308-8146
年卷期： 2019 年 276 卷
页码：
收录情况： SCI
摘要： The availability of analytical methods for quantification of allergens is crucial for the correct assessment and labeling of products in order to protect allergic consumers. For this purpose, a simple, sensitive and accurate technique was developed based on liquid chromatography-tandem mass spectrometry and multiple reaction monitoring mass spectrometry (LC-MRM-MS/MS). The proposed method uses a simple purification with heat and a completely optimized tryptic digestion. This method has been validated according to the requirements defined by ICH (Q2 [R1]), having a linear range from 0.10 to 1179.36 nM with r > 0.999. The parvalbumin beta in flounder (Paralichthys olivaceus) has been quantified at a low level down to 0.10 mu g/g with satisfactory precision (RSD < 18.35%) and accuracy (< 13.3%). The new approach was successfully applied for the determination of parvalbumin beta in the other food matrices.
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