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Identification and Functional Characterization of the DcF3'H Promoter from Dracaena cambodiana

文献类型： 外文期刊

作者： Zhu, Jiahong 1 ; Chen, Pan 1 ; Guo, Dong 1 ; Li, Huiliang 1 ; Wang, Ying 1 ; Dai, Haofu 1 ; Mei, Wenli 1 ; Peng, Shiqing 2 ;

作者机构： 1.Chinese Acad Trop Agr Sci, Inst Trop Biosci & Biotechnol, Minist Agr, Key Lab Trop Crop Biotechnol, Haikou 571101, Hainan, Peoples R China

2.Chinese Acad Trop Agr Sci, Inst Trop Biosci

关键词： Dracaena cambodiana; Dragon's blood; Flavonoid 3-hydroxylase; Promoter

期刊名称：TROPICAL PLANT BIOLOGY （ 影响因子：1.512； 五年影响因子：2.011 ）

ISSN： 1935-9756

年卷期： 2018 年 11 卷 3-4 期

页码：

收录情况： SCI

摘要： Dragon's blood is a traditional medicine that is produced mainly from Dracaenas plants and is widely used in the world. The main chemical compounds in dragon's blood are flavonoids. Flavonoid 3-hydroxylase (F3'H) is an enzyme that synthesizes 3-hydroxylated flavonoids. DcF3'H plays a vital role in determining the flavonoid content during the production of dragons blood; however, the regulation of DcF3'H expression in Dracaenas plants is poorly understood. In this study, a 759-bp promoter region of DcF3'H was isolated from Dracaena cambodiana. The promoter sequence contained cis-acting elements related to various environmental stresses and hormones responses. Dual-luciferase assays showed that the DcF3'H promoter was up-regulated by 6-benzyl aminopurine (6-BA) and down-regulated by methyl jasmonate (MeJA), and these results were consistent with transcription levels of DcF3'H in stems that were verified by quantitative real-time PCR (qPCR). DcHLH1, a bHLH transcription factor identified in yeast one-hybrid screening, was capable of both binding to the promoter and activating the transcription of DcF3'H by similar to 10-fold. The results provide new insights into the regulatory mechanisms of DcF3'H in Dracaena cambodiana.

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