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The putative beta-glucosidase BGL3I regulates cellulase induction in Trichoderma reesei

文献类型： 外文期刊

作者： Zou, Gen 1 ; Jiang, Yanping 1 ; Liu, Rui 1 ; Zhu, Zhihua 1 ; Zhou, Zhihua 1 ;

作者机构： 1.Chinese Acad Sci, CAS Ctr Excellence Mol Plant Sci, Inst Plant Physiol & Ecol, Shanghai Inst Biol Sci,CAS Key Lab Synth Biol, 300 Fenglin Rd, Shanghai 200032, Peoples R China

2.Shanghai Acad Agr Sci, Shanghai Key Lab Agr Genet & Breeding, 1000 Jinqi Rd, Shanghai 201403, Peoples R China

3.Shanghai Acad Agr Sci, Inst Edible Fungi, 1000 Jinqi Rd, Shanghai 201403, Peoples R China

4.Southwest Jiaotong Univ, Chengdu 611756, Sichuan, Peoples R China

关键词： beta-Glucosidase; Cellulase; Induction; Regulation; Trichoderma reesei

期刊名称：BIOTECHNOLOGY FOR BIOFUELS （ 影响因子：6.04； 五年影响因子：6.485 ）

ISSN： 1754-6834

年卷期： 2018 年 11 卷

页码：

收录情况： SCI

摘要： BackgroundThe filamentous fungus Trichoderma reesei (anamorph of Hypocrea jecorina) displays increased cellulase expression while growing on inducers such as lactose or cellulose. However, the mechanism of cellulase induction in T. reesei is not yet completely characterized. Here, a protein annotated as -glucosidase (BGL3I) was found to be involved in cellulase induction in T. reesei. The effects of BGL3I on cellulase production have not yet been fully understood.ResultsDeletion of the bgl3i gene had no influence on the growth of T. reesei, but significantly increased its cellulase activities. Deletion of bgl3i also resulted in decreased extracellular galactosidase activity, but significantly increased transcription of lactose permeases, which might be involved in lactose transport. Furthermore, deletion of bgl3i enhanced the transcription levels of intracellular -glucosidases cel1a, cel1b and the regulator xyr1, which are all essential for lactose induction in T. reesei. BGL3I was found to have a relatively high ability to hydrolyze sophorose, which is proposed to be the strongest natural inducer of cellulase synthesis in T. reesei.ConclusionsBGL3I may take part in the complex regulating system of cellulase induction. The deletion of bgl3i offers a new strategy to improve T. reesei strain performance.

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