您好，欢迎访问中国水产科学研究院 机构知识库！
中国水产科学研究院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

De novo assembly of genome and development of polymorphic microsatellite loci in the blue swimming crab (Portunus pelagicus) using RAD approach

文献类型： 外文期刊

作者： Wu, Qingyang 1 ; Miao, Guidong 1 ; Li, Xincang 4 ; Liu, Wenhua 1 ; Ikhwanuddin, Mhd 2 ; Ma, Hongyu 1 ;

作者机构： 1.Shantou Univ, Guangdong Prov Key Lab Marine Biotechnol, 243 Daxue Rd, Shantou 515063, Peoples R China

2.Shantou Univ, STU UMT Joint Shellfish Res Lab, Shantou 515063, Peoples R China

3.Xingyi Normal Univ Nationalities, Coll Biol & Chem, Xingyi 562400, Peoples R China

4.Chinese Acad Fishery Sci, East China Sea Fisheries Res Inst, Shanghai 200090, Peoples R China

5.Univ Malaysia Terengganu, Inst Trop Aquaculture, Kuala Terengganu 21030, Malaysia

关键词： Portunus pelagicus; Molecular marker; Restriction-site associated DNA sequencing; Polymorphic microsatellites

期刊名称：MOLECULAR BIOLOGY REPORTS （ 影响因子：2.316； 五年影响因子：2.357 ）

ISSN： 0301-4851

年卷期： 2018 年 45 卷 6 期

页码：

收录情况： SCI

摘要： The blue swimming crab (Portunus pelagicus) is a valuable marine fishery resource in Indo-West Pacific Ocean. So far, rare genetic resource of this species is available. In this report, the restriction-site associated DNA (RAD) approach was employed to mine the genomic information and identify molecular markers in P. pelagicus. A total of 0.82 Gbp clean data were generated from the genome of individual "X2A". De novo assembly produced 85,796 contigs with an average length of 339 bp. A total of 45,464 putative SNPs and 17,983 microsatellite loci were identified from the genomes of ten individuals. Furthermore, 31 pairs of primers were successfully designed, with 16 of them exhibiting polymorphism in a wild population. For these polymorphic loci, the expected and observed alleles per locus ranged from 1.064 to 7.314 and from 2 to 11, respectively. The expected and observed heterozygosity per locus ranged from 0.0615 to 0.819 and from 0.0626 to 1.000, respectively. Nine loci showed high informative with polymorphism information content (PIC) > 0.5. Five loci significantly deviated from Hardy-Weinberg equilibrium in the samples analyzed. No linkage disequilibrium was found among the 16 polymorphic microsatellite loci. This study provided massive genetic resource and polymorphic molecular markers that should be helpful for studies on conservation genetics, population dynamics and genetic diversity of P. pelagicus and related crab species.

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：中国水产科学研究院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台