Rapid subtyping of pathogenic and nonpathogenic Vibrio parahaemolyticus by fourier transform infrared spectroscopy with chemometric analysis
文献类型： 外文期刊
作者： Li, Zhaojie 1 ; Chen, Shouguang 1 ; Xu, Chenggang 1 ; Ju, Lingyan 1 ; Li, Fengling 2 ;
作者机构： 1.Weihai Exit & Entry Inspect & Quarantine Bur, Weihai 264200, Shandong, Peoples R China
2.Chinese Acad Fishery Sci, Yellow Sea Fishery Res Inst, Minist Agr, Key Lab Testing & Evaluat Aquat Prod Safety & Qua, Qingdao 266071, Shandong, Peoples R China
关键词： Vibrio parahaemolyticus; Subtype; Pathogenicity; Fourier transform infrared spectroscopy; Principal component analysis; Hierarchical cluster analysis
期刊名称：JOURNAL OF MICROBIOLOGICAL METHODS （ 影响因子：2.363； 五年影响因子：2.24 ）
ISSN： 0167-7012
年卷期： 2018 年 155 卷
页码：
收录情况： SCI
摘要： Vibrio parahaemolyticus which naturally inhabits marine and estuarine environment represents pathogenic strains (virulence genes tdh or trh positive) and non-pathogenic strains (virulence genes negative). In this study, a rapid method for subtyping pathogenic and non-pathogenic V. parahaemolyticus was established using fourier transform infrared (FTIR) spectroscopy with chemometric analysis. This method targeted three strains of genotypes of V. parahaemolyticus including tdh positive, trh positive and virulence gene-negative (nonpathogenic) V. parahaemolyticus. The FTIR absorption spectra between 1800 and 900 cm(-1) highlighted the most distinctive variations and were the most useful for characterizing the three bacteria. The successful differentiation and identification of the three bacteria could be accomplished in less than 1 h by FTIR using principal component analysis (PCA), or another cluster model of hierarchical cluster analysis (HCA). The method was verified by analyzing spiked V. parahaemolyticus fish samples. Furthermore, all of ten clinical isolates of V. parahaemolyticus were identified as tdh-positive, none of the clinical isolates were n h-positive, and all of ten environmental isolates were identified as non-pathogenic by the subtyping method, which were confirmed by PCR assays. All data demonstrated that the newly established subtyping method by FTIR is practical, time-saving, labor-saving, specific and cost-effective, especially suitable for the basic laboratories of CDC and port quarantine departments to perform suiveillance and epidemiological traceability of pathogenic V. parahaemolyticus.
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