Development and Evaluation of Real-Time Polymerase Chain Reaction (PCR) to Authenticate Sablefish (Anoplopoma fimbria) in Commercial Seafood Products
文献类型： 外文期刊
作者： Qu, Meng 1 ; Jiang, Yanhua 1 ; Guo, Yingying 1 ; Zhu, Wenjia 1 ; Liu, Shufang 2 ; Li, Na 1 ; Li, Fengling 1 ; Yao, Lin 1 ; Tan, Zhijun 1 ; Wang, Lianzhu 1 ;
作者机构： 1.Minist Agr & Rural Affairs, Key Lab Testing & Evaluat Aquat Prod Safety & Qual, Qingdao, Peoples R China
2.Yellow Sea Fisheries Res Inst, Chinese Acad Fishery Sci, Qingdao, Peoples R China
3.Minist Agr & Rural Affairs, Key Lab Sustainable Dev Marine Fisheries, Qingdao, Peoples R China
4.Minist Agr & Rural Affairs, Key Lab Testing & Evaluat Aquat Prod Safety & Qual, 106 Nanjing Rd, Qingdao 266071, Shandong, Peoples R China
关键词： Sablefish; real-time polymerase chain reaction; authentication; cytochrome b gene
期刊名称：JOURNAL OF AQUATIC FOOD PRODUCT TECHNOLOGY （ 影响因子：1.6； 五年影响因子：1.9 ）
ISSN： 1049-8850
年卷期： 2023 年 32 卷 10 期
页码：
收录情况： SCI
摘要： By comparing the cytochrome b fragment sequences of sablefish and 16 varieties susceptible to adulteration, a real-time polymerase chain reaction (PCR) method was established to detect sablefish-derived components. The PCR method was used to analyze commercial products. The method developed was highly specific without any cross-reaction in the other 16 species and with a limit of detection for deoxyribonucleic acid (DNA) of 0.002 ng mu L-1 for sablefish. In addition, the mixed muscle tissues were detected at as low as 0.01 g kg(-1). The applicability of the PCR method to 50 commercial prepackaged products revealed the accuracy of this method.
- 相关文献
作者其他论文 更多>>
-
First record of abnormal body coloration in a rockfish Sebastes koreanus (Scorpaenoidei: Sebastidae) from coastal water of China based on morphological characteristics and DNA barcoding
作者：Li, Ang;Wang, Huan;An, Changting;Liu, Shufang;Li, Ang;Liu, Shufang
关键词：abnormal body coloration; Sebastes koreanus; coastal water of China; Yellow Sea; morphological characteristics; DNA barcoding
-
A novel ZIF-L/PEI thin film nanocomposite membrane for removing perfluoroalkyl substances (PFASs) from water: Enhanced retention and high flux
作者：Bi, Yujie;Meng, Xiangmin;Yong, Qiaozhi;Wang, Xinping;Tan, Zhijun;Geng, Qianqian;Peng, Jixing;Sun, Xiaojie;Guo, Mengmeng
关键词：Perfluoroalkyl substances (PFASs); Thin film nanocomposite (TFN); Metal-organic framework (MOF); Density functional theory (DFT); Nanofiltration (NF)
-
Integrated evidence of transcriptional, metabolic, and intestinal microbiota changes in Ruditapes philippinarum due to perfluorooctanoic acid-induced immunotoxicity
作者：Li, Fengling;Gong, Xiuqiong;Zhou, Yang;Geng, Qianqian;Jiang, Yanhua;Yao, Lin;Qu, Meng;Tan, Zhijun;Gong, Xiuqiong;Zhou, Yang;Tan, Zhijun
关键词：Perfluorooctanoic acid; Immunotoxicity; Intestine microbiota; Metabolome; Transcriptome; Bivalve
-
Contamination Status and Risk Assessment of Paralytic Shellfish Toxins in Shellfish along the Coastal Areas of China
作者：Zheng, Guanchao;Xu, Xizhen;Wu, Haiyan;Fan, Liqiang;Peng, Jixing;Guo, Mengmeng;Tan, Zhijun;Xu, Xizhen;Wang, Qianrui;Yang, Dajin;Tan, Zhijun
关键词：paralytic shellfish toxins; shellfish; liquid chromatography-tandem mass spectrometry (LC-MS/MS); risk assessment; China
-
Influence of humic acid on the bioaccumulation, elimination, and toxicity of PFOS and TBBPA co-exposure in Mytilus unguiculatus Valenciennes
作者：Geng, Qianqian;Zou, Liang;Guo, Mengmeng;Li, Fengling;Qin, Hanlin;Tan, Zhijun;Geng, Qianqian;Liu, Xiangxiang;Wang, Xu;Zou, Liang;Liu, Hong;Wang, Xu;Tan, Zhijun
关键词：Perfluoroalkyl acids; Brominated flame retardant; Dissolved organic matter; Bioconcentration; Mussel; Co-exposure
-
Immune and physiological responses of Mytilus unguiculatus to Alexandrium spp. with varying paralytic shellfish toxin profiles
作者：Wu, Haiyan;Zhang, Qianru;Zheng, Guanchao;Geng, Qianqian;Tan, Zhijun;Wu, Haiyan;Tan, Zhijun;Yang, Yuecong
关键词：Total PSTs content; Total hemocyte count; Immunity; Nucleotide imbalance; Bivalve
-
Integrative Inducer Intervention and Transcriptomic Analyses Reveal the Metabolism of Paralytic Shellfish Toxins in Azumapecten farreri
作者：Dong, Chenfan;Zheng, Guanchao;Peng, Jixing;Guo, Mengmeng;Wu, Haiyan;Dong, Chenfan;Tan, Zhijun;Tan, Zhijun
关键词：cytochrome P450; glutathione S-transferase; ATP-binding cassette transporter; rifampin; dl-alpha-tocopherol; colchicine
