Development and Evaluation of Real-Time Polymerase Chain Reaction (PCR) to Authenticate Sablefish (Anoplopoma fimbria) in Commercial Seafood Products
文献类型： 外文期刊
作者： Qu, Meng 1 ; Jiang, Yanhua 1 ; Guo, Yingying 1 ; Zhu, Wenjia 1 ; Liu, Shufang 2 ; Li, Na 1 ; Li, Fengling 1 ; Yao, Lin 1 ; Tan, Zhijun 1 ; Wang, Lianzhu 1 ;
作者机构： 1.Minist Agr & Rural Affairs, Key Lab Testing & Evaluat Aquat Prod Safety & Qual, Qingdao, Peoples R China
2.Yellow Sea Fisheries Res Inst, Chinese Acad Fishery Sci, Qingdao, Peoples R China
3.Minist Agr & Rural Affairs, Key Lab Sustainable Dev Marine Fisheries, Qingdao, Peoples R China
4.Minist Agr & Rural Affairs, Key Lab Testing & Evaluat Aquat Prod Safety & Qual, 106 Nanjing Rd, Qingdao 266071, Shandong, Peoples R China
关键词： Sablefish; real-time polymerase chain reaction; authentication; cytochrome b gene
期刊名称：JOURNAL OF AQUATIC FOOD PRODUCT TECHNOLOGY （ 影响因子：1.6； 五年影响因子：1.9 ）
ISSN： 1049-8850
年卷期： 2023 年 32 卷 10 期
页码：
收录情况： SCI
摘要： By comparing the cytochrome b fragment sequences of sablefish and 16 varieties susceptible to adulteration, a real-time polymerase chain reaction (PCR) method was established to detect sablefish-derived components. The PCR method was used to analyze commercial products. The method developed was highly specific without any cross-reaction in the other 16 species and with a limit of detection for deoxyribonucleic acid (DNA) of 0.002 ng mu L-1 for sablefish. In addition, the mixed muscle tissues were detected at as low as 0.01 g kg(-1). The applicability of the PCR method to 50 commercial prepackaged products revealed the accuracy of this method.
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