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Genome-wide identification of auxin-responsive microRNAs in the poplar stem

文献类型： 外文期刊

作者： Yang, Lihua 1 ; Ping, Tao 2 ; Lu, Wenjin 2 ; Song, Sangfa 2 ; Wang, Jianli 3 ; Wang, Qiao 2 ; Chai, Guohua 2 ; Bai, Yue 5 ; Chen, Yan 5 ;

作者机构： 1.Inner Mongolia Agr Univ, Coll Life Sci, Hohhot 010018, Peoples R China

2.Qingdao Agr Univ, Coll Resources & Environm, Qingdao 266109, Peoples R China

3.Heilongjiang Acad Agr Sci, Grass & Sci Inst, Harbin 150086, Peoples R China

4.Qingdao Agr Univ, Acad Dongying Efficient Agr Technol & Ind Saline &, Dongying 257000, Peoples R China

5.Inner Mongolia Agr Univ, Forestry Coll, Hohhot 010018, Peoples R China

6.Qingdao Agr Univ, Coll Landscape Architecture & Forestry, Qingdao 266109, Peoples R China

关键词： MicroRNA; Auxin; Wood; Populus

期刊名称：GENES & GENOMICS （ 2022影响因子：2.1； 五年影响因子：1.9 ）

ISSN： 1976-9571

年卷期： 2023 年 45 卷 8 期

页码：

收录情况： SCI

摘要： BackgroundWood (secondary xylem) of forests is a material of great economic importance. Wood development is strictly controlled by both the phytohormone auxin and microRNAs (miRNAs). Currently, the regulatory mechanisms underlying wood formation by auxin-associated miRNAs remain unclear.ObjectiveThis report was designed to identify auxin-responsive miRNAs during wood formation.MethodsMorphological observation of wood development in the poplar stems was performed under the treatment of different concentrations (0 mg/L, CK; 5 mg/L, Low; 10 mg/L, High) of indol-3-butyric acid (IBA). Using a small RNA sequencing strategy, the effect of IBA treatment on miRNAs expression was genome-widely analyzed.ResultsIn this study, we found that wood development of poplar was promoted by low concentration of IBA treatment but inhibited by high concentration of IBA treatment. Stringent bioinformatic analysis led to identification of 118 known and 134 novel miRNAs candidates. Sixty-nine unique developmental-related miRNAs, corresponding to 269 target genes, exhibited specific expression patterns in response to auxin, as was consistent with the influence of auxin application on wood formation. Three novel miRNAs had the most number (>= 9) of target genes, belonging to SPL, GRF and ARF gene families. The evolutionary relationships and tissue expression patterns of 41 SPL, GRF and ARF genes in poplar were thus analyzed. Of them, four representative members and corresponding miRNAs were confirmed using RT-qPCR.ConclusionsOur results may be helpful for a better understanding of auxin-induced regulation of wood formation in tree species.

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