A lateral flow assay for copper(II) utilizing catalytic and stem-loop based signal amplification
文献类型： 外文期刊
作者： Wang, Yulong 1 ; Wang, Limin 3 ; Zhang, Cunzheng 1 ; Liu, Fengquan 2 ;
作者机构： 1.Jiangsu Acad Agr Sci, Key Lab Control Technol & Stand Agroprod Safety &, Key Lab Food Qual & Safety Jiangsu Prov, State Key Lab Breeding Base,Minist Agr, Nanjing 210014, Jiangsu, Peoples R China
2.Jiangsu Acad Agr Sci, Inst Plant Protect, Jiangsu Key Lab Food Qual & Safety, State Key Lab Cultivat Base,Minist Sci & Technol, Nanjing 210014, Jiangsu, Peoples R China
3.Nanjing Agr Univ, Coll Plant Protect, Key Lab Integrated Management Crop Dis & Pests, Nanjing 210095, Jiangsu, Peoples R China
关键词： Target recycling; DNAzyme; Gold nanoparticle; Semiquantitative; Visual detection; Copper ions
期刊名称：MICROCHIMICA ACTA （ 影响因子：5.833； 五年影响因子：5.357 ）
ISSN： 0026-3672
年卷期： 2019 年 186 卷 2 期
页码：
收录情况： SCI
摘要： A DNAzyme-based catalytic and stem-loop based amplification scheme is used in a Cu(II)-specific lateral flow assay (LFA). Three test lines with given cut-off value on the test strip are set as the signal indicating zone for semiquantitative analysis by the number of red color bands that appear after lateral flow. The colored bands are generated by accumulation of gold nanoparticles. Four detection ranges can be visualied: (a) 0-2ngmL(-1) (= negative); 2-50ngmL(-1); 50-200ngmL(-1) and>200ngmL(-1) of Cu(II) (= positive). The visual detection limit is thus considered as being 2ngmL(-1) which is much lower than the U.S. EPA limit in drinking water (1.25gmL(-1)). The highly specific DNAzyme, the strong multiple-turnover catalytic target recycling property and highly efficient amplification strategy warrant the high specificity, sensitivity and rapidity of this LFA. Conceivbly, this detecton scheme can be extended to other metal ions by proper choice of the ion-specific DNAzyme.
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