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Immunochromatographic Assay for Maximal Residual Limit-based Multiple Quantitative Determination of Triazophos in Agro Products

文献类型： 外文期刊

作者： Gong Hang 1 ; Liu Bei-Bei 2 ; Li Pan 2 ; He Dan 1 ; Guo Yi-Rong 3 ; Wang Li-Min 4 ; Hua Xiu-De 4 ; Wang Ming-Hua 5 ; Liu Fen 1 ;

作者机构： 1.Anhui Sci & Technol Univ, Coll Food & Drug, Fengyang 233100, Peoples R China

2.Jiangsu Acad Agr Sci, Inst Food Safety & Nutr, Nanjing 210014, Jiangsu, Peoples R China

3.Zhejiang Univ, Inst Pesticide & Environm Toxicol, Hangzhou 310058, Zhejiang, Peoples R China

4.Nanjing Agr Univ, Coll Plant Protect, Nanjing 210095, Jiangsu, Peoples R China

5.Na

关键词： Triazophos; Immunochromatographic assay; Maximal residual limit value; Rapid detection

期刊名称：CHINESE JOURNAL OF ANALYTICAL CHEMISTRY （ 影响因子：1.134； 五年影响因子：0.909 ）

ISSN： 0253-3820

年卷期： 2018 年 46 卷 6 期

页码：

收录情况： SCI

摘要： A novel immunochromatographic assay was developed, which could provide visual evidence of triazophos in agro products, and also could directly identify the safety status by setting visual cut-off limit of detection in maximal residual limit (MRL) value. Three test lines (T1, T2, T3) were applied to the nitrocellulose membrane with different concentrations of Triazophos-OVA, and one control line (C) was settled with goat anti mouse IgG antibody. Thereafter, by combining with conjugate pad which immobilized monoclonal antibody labeled with 20 nm Colloidal gold particles, absorbent pad and PVC plate, a chromatographic test strip was assembled. With optimization of sample extraction and solvents selection, the test strips were employed for the determination of triazophos in rice, cabbage and apple. The results revealed that the cut-off limit of detection could reach 0.005, 0.01 and 0.02 mu g/mL represented by test line T3, T2 and T1, respectively. After modification, the cut-off limit of detection was resettled to 0.05, 0.1 and 0.2 mu g/mL according to the MRL values which enforced by the national standard of GB2763. Using acetonitrile for the sample extraction, the extracts were diluted 10 times or solvent exchanged with equivalent volume by PBS solution, and then tested by strips descripted above mentioned. The two test strips could precisely identified the safety status of agro product with MRL as threshold within 8-12 min. Furthermore, the residues value of triazophos could be quantified by the multiple quantitative test lines. Parallel GC data indicated that the strip had no false negative. This MRL-based multiple quantitative triazophos detection strip would provide a simple, direct, accurate and the most intuitionistic performance for the evaluation of agro product safety.

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