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An Indirect Enzyme-Linked Immunosorbent Assay for Determination of New Herbicide H-9201 in Water

文献类型： 外文期刊

作者： Zhang Cun-Zheng 1 ; Yang Chun-Long 3 ; Liu Xian-Jin 1 ; Chen Min 3 ; Yu Jie 1 ;

作者机构： 1.Minist Agr, Key Lab Food Safety Monitoring & Management, Nanjing 210014, Peoples R China

2.Jiangsu Prov State Key Lab Breeding Base, Key Lab Food Qual & Safety, Nanjing 210014, Peoples R China

3.Nanjing Agr Univ, Coll Sci, Nanjing 210095, Peoples R China

关键词： Herbicide;H-9201;Enzyme-linked immunosorbent assay;Polyclonal antibody

期刊名称：CHINESE JOURNAL OF ANALYTICAL CHEMISTRY （ 影响因子：1.134； 五年影响因子：0.909 ）

ISSN： 0253-3820

年卷期： 2011 年 39 卷 2 期

页码：

收录情况： SCI

摘要： A sensitive indirect enzyme-linked immunosorbent assay (ELISA) was developed for the detection of new herbicide H-9201, and their suitability for the determination of this analyte in environmental water was studied. Starting with phosphorus thiochloride and methanol, an analogs with similar structure of hapten was synthesized, and carboxyl group was conducted by succinic anhydride, then the derivative was conjugated with bovine serum albumin or ovalbumin by means of active ester as immunoagent and coating agent, respectively; IC-ELISA method was developed based on the polyclonal antibody generated in rabbits by immunizing with synthesized hapten. For standard, 50% reduction of the maximum ELISA signal (IC50) was 1. 331 mg/L, whereas the detection limit was 0. 017 mg/L in the competitive standard curve ranged from 0. 05 to 20. 0 mg/L and the combination of the concentrations of coating solution and primary antibody was optimized to 1/512 and 1/18000, respectively. No significant cross-reactivity for related compounds was observed, such as 2,4-dimethyl-6-nitrophenol, p-nitrophenol, Methamidophos, phorate, chlorpyrifos, parathion, the antibody showed negligible cross-reactivity with S-carboxylethyl-O, O-Dimethyl-phosphorodithioate, dimethoate, malathion, but high cross-reactivity with analogs of hapten. Recovery of H-9201 in environmental water was (80.5 +/- 4. 5)%-(95. 2 +/- 3. 2)% based on the developed IC-ELISA method.

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