您好，欢迎访问江苏省农业科学院 机构知识库！
江苏省农业科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Lateral Flow Immunoassay for Simultaneous Determination of Four Kinds of Pyrethroid Pesticides in Vegetables and Fruits

文献类型： 外文期刊

作者： Hu Jing 1 ; Guo Yi-Rong 2 ; Liang Xiao 1 ; Liu Xian-Jin 1 ; Zhu Guo-Nian 2 ; Liu Feng-Quan 3 ; Wang Ming-Hua 4 ; Wang Li- 1 ;

作者机构： 1.Jiangsu Acad Agr Sci, Inst Food Safety, Nanjing 210014, Jiangsu, Peoples R China

2.Zhejiang Univ, Inst Pesticide & Environm Toxicol, Hangzhou 310058, Zhejiang, Peoples R China

3.Jiangsu Acad Agr Sci, Inst Plant Protect, Nanjing 210014, Jiangsu, Peoples R China

4.Nanjing Agr Univ, Coll Plant Protect, Nanjing 210095, Jiangsu, Peoples R China

关键词： Pyrethroid pesticide;Lateral flow immunoassay;Rapid detection

期刊名称：CHINESE JOURNAL OF ANALYTICAL CHEMISTRY （ 影响因子：1.134； 五年影响因子：0.909 ）

ISSN： 0253-3820

年卷期： 2016 年 44 卷 12 期

页码：

收录情况： SCI

摘要： A monoclonal antibody based lateral flow immunoassay was developed for the simultaneous determination of four pyrethroid pesticides in vegetables and fruits, including fenpropathrin, cypermethrin, deltamethrin, and cyhalothrin. The colloidal gold particles (20-nm) were prepared by the method of sodium citrate reduction and conjugated with goat anti. mice IgG. Antigen and rabbit anti. goat IgG were immobilized on the nitrocellulose membrane as the test line (T line) and the control line (C line), separately. By sticking on PVC board, a gold immunochromatographic strip was assembled. About 10 g of samples was extracted by acetonitrile, and then diluted four times with PBS buffer. Anti. pyrethroid McAb was added in 100 mu L of this extract solution for the antibody. antigen binding, thereafter, the mixture was ready for the strip testing. The results revealed that the test could be done within 10 min, and the cutoff values reached 0. 5, 5. 0, 5. 0, 5. 0 mu g/mL for fenpropathrin, cypermethrin, deltamethrin, cyhalothrin, respectively. If the extraction going through QuEChERS method, the detection limit of method could be improved 16 times. Thereafter, the lateral flow immunoassay was employed for the verification test with 13 types of vegetables and fruits, except that the result of chillies presented the false positive result, the other results obtained higher consistency with the results of GC test. This new format of test strip using colloidal gold labeled goat anti. mice IgG as tracer, which could give more stable and repeatable results than classic test strip, would provide a new method for the simultaneous determination of four pyrethroid pesticides in vegetables and fruits.

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：江苏省农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台