您好，欢迎访问江苏省农业科学院 机构知识库！
江苏省农业科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Determination of Dimethachlon in Tobacco by Time-resolved Fluorescence Immunoassay

文献类型： 外文期刊

作者： Liu Yuan 1 ; Liu Rong-Rong 1 ; Liu Xian-Jin 1 ; Fang Dun-Huang 2 ; Song Cun-Man 2 ; Huovinen, Tuomas 3 ; Vehnianen, Ma 1 ;

作者机构： 1.Jiangsu Acad Agr Sci, Inst Food Safety, Nanjing 210014, Jiangsu, Peoples R China

2.Yunnan Inst Tobacco, Yuxi 653100, Peoples R China

3.Univ Turku, Turku 20540, Finland

关键词： Dimethachlon;Time-resolved fluorescence immunoassay;Tobacco;Sample pretreatment

期刊名称：CHINESE JOURNAL OF ANALYTICAL CHEMISTRY （ 影响因子：1.134； 五年影响因子：0.909 ）

ISSN： 0253-3820

年卷期： 2012 年 40 卷 7 期

页码：

收录情况： SCI

摘要： A sensitive time-resolved fluorescence immunoassay (TRFIA) with a cleanup-free pretreatment for dimethachlon in tobacco was developed. The competitive indirect TRFIA for dimethachlon was established by using goat anti-rabbit IgG conjugated with a Eu-N1 clelate as a tracer. The detection limit (I-10) and I-50 for this assay was 1.98 mu g/L and 32.00 mu g/L, respectively. And the linear range for detection is between 3.97-128.76 mu g/L. Meanwhile, the effect of tobacco matrix to TRFIA was also studied. It was demonstrated that the linear range of inhibition curve with 1% tobacco matrix was parallel to the standard curve; so the extract of tobacco would be diluted 100 times before assay, the index of matrix interference (I-m) was calculated as 17.1. Three levels of dimethachon (1, 7 and 24 mg/L) were spiked into tobacco, the overall recoveries and the relative standard deviations (RSDs) were in the range of 73%-128% and 4.3-13.2, and the practical detection limit for the method was 1 mg/L. This assay is a suitable tool for screening dimethachlon residue in tobacco samples

  • 相关文献

[1]Cloning and expression of a wild eggplant cytochrome P450 gene, StoCYP77A2, involved in plant resistance to Verticillium dahliae. Yang, Liu,Shi, Ce,Mu, Xiaoying,Liu, Chao,Shi, Ke,Zhu, Wenjiao,Yang, Qing,Yang, Liu.

[2]Overexpression of the CaTIP1-1 Pepper Gene in Tobacco Enhances Resistance to Osmotic Stresses. Yin, Yan-Xu,Xiao, Huai-Juan,Zhang, Huai-Xia,Zhang, Zhen,Jing, Hua,Zhang, Ying-Li,Chen, Ru-Gang,Gong, Zhen-Hui,Wang, Shu-Bin,Zhang, Ying-Li. 2014

[3]A CC-NBS-LRR type gene GHNTR1 confers resistance to southern root-knot nematode in Nicotiana.benthamiana and Nicotiana.tabacum. Zhang, Baolong,Zhang, Wenhua,Zhang, Baolong,Yang, Yuwen,Wang, Jinyan,Ling, Xitie,Hu, Zhongze,Liu, Tingli,Chen, Tianzi.

[4]Thymol Mitigates Cadmium Stress by Regulating Glutathione Levels and Reactive Oxygen Species Homeostasis in Tobacco Seedlings. Ye, Xiefeng,Ling, Tianxiao,Xue, Yanfeng,Xu, Cunfa,Zhou, Wei,Hu, Liangbin,Chen, Jian,Shi, Zhiqi,Chen, Jian,Shi, Zhiqi.

[5]Cinnamaldehyde Ameliorates Cadmium-Inhibited Root Elongation in Tobacco Seedlings via Decreasing Endogenous Hydrogen Sulfide Production. Ye, Xie-Feng,Ling, Tianxiao,Yu, Xiao-Na,Xue, Yanfeng,Wang, Yong,Cheng, Changxin,Feng, Guosheng,Hu, Liangbin,Shi, Zhiqi,Chen, Jian,Shi, Zhiqi,Chen, Jian.

作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：江苏省农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台