Isolation, identification, and classification of a novel rhabdovirus from diseased Chinese rice-field eels (Monopterus albus)
文献类型： 外文期刊
作者： Liu, Wenzhi 1 ; Fan, Yuding 1 ; Li, Zhong 1 ; Zhao, Jianqing 1 ; Zhou, Yong 1 ; Jiang, Nan 1 ; Zeng, Jia 1 ; Cain, Kennet 1 ;
作者机构： 1.Chinese Acad Fishery Sci, Yangtze River Fisheries Res Inst, Wuhan 430223, Hubei, Peoples R China
2.Huazhong Agr Univ, Coll Fisheries, Wuhan 430070, Peoples R China
3.Univ Idaho, Dept Fish & Wildlife Sci, Moscow, ID 83843 USA
4.Univ Idaho, Inst Aquaculture Res, Moscow, ID 83843 USA
期刊名称：ARCHIVES OF VIROLOGY （ 影响因子：2.574； 五年影响因子：2.466 ）
ISSN： 0304-8608
年卷期： 2019 年 164 卷 1 期
页码：
收录情况： SCI
摘要： In 2017, a clinical disease outbreak resulted in substantial mortality of adults and larvae of cultured Chinese rice-field eels (Monopterus albus) on a farm in Hubei, Central China. A rhabdovirus was isolated from moribund specimens, and typical clinical symptoms associated with an outbreak included an enlarged and swollen head. This differed from previous observations. Histological changes included necrosis and cavities of various sizes within the brain and kidney. Homogenized tissues of diseased Chinese rice-field eels were screened for viral isolation using six different fish cell lines. A rhabdovirus was isolated following observation of cytopathic effect (CPE) in a gibel carp brain (GiCB) cell line and confirmed by RT-PCR. Electron microscopy showed large numbers of rhabdovirus-shaped particles in the cytoplasm of the brain cells of the diseased Chinese rice-field eels and in the infected GiCB cell line. This virus has been named Chinese rice-field eel rhabdovirus (CrERV), and the complete nucleotide sequence of CrERV was cloned. This rhabdovirus is composed of 11,545 nucleotides with the following genomic organization: 3-N-P-M-G-L-5. The genes are separated by conserved gene junctions, and phylogenetic analysis of the L sequence revealed that CrERV forms a separate branch with Siniperca chuatsi rhabdovirus (SCRV) and hybrid snakehead rhabdovirus C1207 (HSHRV-C1207). This is the first report of the complete sequence of CrERV from the Chinese rice-field eel in China.
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