Rapid detection of Cucumber green mottle mosaic virus by reverse transcription recombinase polymerase amplification
文献类型： 外文期刊
作者： Zeng, Rong 1 ; Luo, Jinyan 2 ; Gao, Shigang 1 ; Xu, Lihui 1 ; Song, Zhiwei 1 ; Dai, Fuming 1 ;
作者机构： 1.Shanghai Acad Agr Sci, Inst Ecoenvironm & Plant Protect, Shanghai Key Lab Protect Hort Technol, Shanghai 201403, Peoples R China
2.Shanghai Extens & Serv Ctr Agr Tech, Shanghai 201103, Peoples R China
关键词： CGMMV; Isothermal amplification; Specificity
期刊名称：MOLECULAR AND CELLULAR PROBES （ 影响因子：2.365； 五年影响因子：2.386 ）
ISSN： 0890-8508
年卷期： 2019 年 43 卷
页码：
收录情况： SCI
摘要： Reverse transcription recombinase polymerase amplification (RT-RPA) for detection of Cucumber green mottle mosaic virus (CGMMV) was developed in this study. It was found to be specific, with a limit of detection of 0.5 pg of total CGMMV RNA. CGMMV on inoculated leaves was tested using RT-RPA assay, suggesting that this method is suitable for CGMMV detection from leaves.
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