Rapid Detection of SdhB(P225F) and Sdhe(H272R )Mutations in Boscalid Resistant Botrytis cinerea Strains by ARMS-PCR
文献类型： 外文期刊
作者： Liu, Xin 1 ; Zeng, Rong 1 ; Gao, Shigang 1 ; Xu, Lihui 1 ; Dai, Fuming 1 ;
作者机构： 1.Shanghai Acad Agr Sci, Inst Ecoenvironm & Plant Protect, Shanghai Key Lab Protect Hort Technol, 1000 Jinqi Rd, Shanghai 201403, Peoples R China
关键词： ARMS-PCR; boscalid resistance; detection of point mutations; grey mould; PASA
期刊名称：PLANT PATHOLOGY JOURNAL （ 影响因子：1.795； 五年影响因子：2.314 ）
ISSN： 1598-2254
年卷期： 2019 年 35 卷 1 期
页码：
收录情况： SCI
摘要： SdhB(P225F) and SdhB(H272R) mutations have been found associated with boscalid resistance in Botrytis cinerea from strawberry in Shanghai, China. For rapid detection of two mutations, tetra-primers were designed and optimized to gain the relatively high accuracy and specificity based on the ARMS-PCR technique, by which resistance can be identified with different lengths of products on agarose gels. The tetra-primer ARMS-PCR systems for SdhB(P225F) and SdhB(H272R) were validated by 9 SdhB-squenced strains repeatedly. Then, sensitivity of 30 more strains were also tested by the methods, which were accordant with genotypes by sequencing and the sensitivity of conidial germination to boscalid by 100%. Thus, the methods developed in this study are proved to be rapid, inexpensive, accurate and practical for resistance detection of Botrytis cinerea caused by SdhB(P225F) and SdhB(H272R) mutations.
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