Rapid Detection of SdhB(P225F) and Sdhe(H272R )Mutations in Boscalid Resistant Botrytis cinerea Strains by ARMS-PCR
文献类型： 外文期刊
作者： Liu, Xin 1 ; Zeng, Rong 1 ; Gao, Shigang 1 ; Xu, Lihui 1 ; Dai, Fuming 1 ;
作者机构： 1.Shanghai Acad Agr Sci, Inst Ecoenvironm & Plant Protect, Shanghai Key Lab Protect Hort Technol, 1000 Jinqi Rd, Shanghai 201403, Peoples R China
关键词： ARMS-PCR; boscalid resistance; detection of point mutations; grey mould; PASA
期刊名称：PLANT PATHOLOGY JOURNAL （ 影响因子：1.795； 五年影响因子：2.314 ）
ISSN： 1598-2254
年卷期： 2019 年 35 卷 1 期
页码：
收录情况： SCI
摘要： SdhB(P225F) and SdhB(H272R) mutations have been found associated with boscalid resistance in Botrytis cinerea from strawberry in Shanghai, China. For rapid detection of two mutations, tetra-primers were designed and optimized to gain the relatively high accuracy and specificity based on the ARMS-PCR technique, by which resistance can be identified with different lengths of products on agarose gels. The tetra-primer ARMS-PCR systems for SdhB(P225F) and SdhB(H272R) were validated by 9 SdhB-squenced strains repeatedly. Then, sensitivity of 30 more strains were also tested by the methods, which were accordant with genotypes by sequencing and the sensitivity of conidial germination to boscalid by 100%. Thus, the methods developed in this study are proved to be rapid, inexpensive, accurate and practical for resistance detection of Botrytis cinerea caused by SdhB(P225F) and SdhB(H272R) mutations.
- 相关文献
作者其他论文 更多>>
-
Utilizing zinc oxide nanoparticles as an environmentally safe biosystem to mitigate mycotoxicity and suppress Fusarium graminearium colonization in wheat
作者：Ibrahim, Ezzeldin;Hafeez, Rahila;Ogunyemi, Solabomi Olaitan;Abdallah, Yasmine;Li, Bin;Ibrahim, Ezzeldin;Xu, Lihui;Nasser, Raghda;Nasser, Raghda;Adel, Al-Shimaa Mohammed;Zhang, Zhen;Shou, Linfei;Wang, Daoze
关键词：ZnONPs; Antifungal mechanisms; DON reduction; Fusarium graminearum colonization; Plant protection
-
First Report of Bacterial Leaf Spot on Muskmelon Caused by Pseudomonas syringae pv. syringae in China
作者：Song, Zhiwei;Gao, Shigang;Zeng, Rong;Gao, Ping;Xu, Lihui;Dai, Fuming;Song, Zhiwei;Gao, Shigang;Zeng, Rong;Gao, Ping;Xu, Lihui;Dai, Fuming;Fang, Qin
关键词：disease development and spread; epidemiology; fruit; prokaryotes; small fruits
-
Mechanisms Underlying the Overlooked Chiral Fungicide-Driven Enantioselective Proliferation of Antibiotic Resistance in Earthworm Intestinal Microbiome
作者：Song, Jiajin;Zheng, Conglai;Qiu, Mengting;Zhang, Zihan;Zhang, Luqing;Yu, Yunlong;Fang, Hua;Shi, Nan;Zhang, Houpu;Zhan, Xiu-ping;Xu, Lihui;Nicolaisen, Mogens
关键词：chiral fungicide; antibiotic resistance gene; earthworm gut; mobilome; horizontal transfer
-
Nitrogen removal performance and mechanism in constructed wetlands under saline conditions: Role of Canna indica inoculated with Piriformospora indica
作者：Cui, Naxin;Cai, Min;Zhang, Xu;Zeng, Rong;Zhou, Li;Chen, Guifa;Zou, Guoyan;Cui, Naxin;Cai, Min;Zhang, Xu;Zeng, Rong;Zhou, Li;Chen, Guifa;Zou, Guoyan;Cui, Naxin;Zeng, Rong
关键词：Phytoremediation; Salt stress; Rhizosphere microbe; Plant uptake; Growth-promoting fungus
-
Resistance Profiles of Botrytis cinerea to Fluxapyroxad from Strawberry Fields in Shanghai, China
作者：Gao, Ping;Zeng, Rong;Gao, Shigang;Xu, Lihui;Song, Zhiwei;Dai, Fuming
关键词：Botrytis cinerea; fluxapyroxad; resistance; SDHI
-
Potential of Trichoderma virens HZA14 in Controlling Verticillium Wilt Disease of Eggplant and Analysis of Its Genes Responsible for Microsclerotial Degradation
作者：Tomah, Ali Athafah;Abd Alamer, Iman Sabah;Khattak, Arif Ali;Ahmed, Temoor;Zhang, Jingze;Li, Bin;Tomah, Ali Athafah;Abd Alamer, Iman Sabah;Ahmed, Temoor;Hatamleh, Ashraf Atef;Al-Dosary, Munirah Abdullah;Ali, Hayssam M.;Wang, Daoze;Xu, Lihui
关键词：T. virens; mycoparasitism; microsclerotial degradation; transcriptome; qPCR; Solanum melongena L.
-
Fertilization of Microbial Composts: A Technology for Improving Stress Resilience in Plants
作者：Ahmed, Temoor;Qi, Yetong;Yao, Yanlai;Qi, Xingjiang;Ahmed, Temoor;Noman, Muhammad;Li, Bin;Shahid, Muhammad;Hussain, Sabir;Masood, Hafiza Ayesha;Masood, Hafiza Ayesha;Xu, Lihui;Ali, Hayssam M.;Negm, Sally;El-Kott, Attalla F.
关键词：biofertilizer; plant diseases; compost; nutrient transformation; PGPR
