Protection of Porcine Intestinal-Epithelial Cells from Deoxynivalenol-Induced Damage by Resveratrol via the Nrf2 Signaling Pathway
文献类型： 外文期刊
作者： Yang, Jun 1 ; Zhu, Cui 2 ; Ye, Jinling 2 ; Lv, Yantao 2 ; Wang, Li 3 ; Chen, Zhuang 2 ; Jiang, Zongyong 2 ;
作者机构： 1.South China Agr Univ, Coll Anim Sci, Guangzhou 510642, Guangdong, Peoples R China
2.Guangdong Acad Agr Sci, Agrobiol Gene Res Ctr, Guangzhou 510640, Guangdong, Peoples R China
3.Guangdong Acad Agr Sci, Inst Anim Sci, Guangzhou 510640, Guangdong, Peoples R China
关键词： resveratrol; deoxynivalenol; IPEC-J2 cells; oxidative damage; Nrf2 signaling pathway
期刊名称：JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY （ 影响因子：5.279； 五年影响因子：5.269 ）
ISSN： 0021-8561
年卷期： 2019 年 67 卷 6 期
页码：
收录情况： SCI
摘要： Deoxynivalenol (DON), a common mycotoxin, usually induces oxidative stress and affects the intestinal health of humans and animals. This study investigated the protective effect of resveratrol (RES), a natural antioxidant, on alleviating the cytotoxicity induced by DON in the porcine intestinal-epithelial cell line (IPEC-J2). Cells were incubated with RES for 24 h and then exposed to DON for another 24 h. Cell viability, proliferation, apoptosis, and oxidative-stress indicators were determined. In comparison with DON-only-treated cells, pretreatment with RES (15 mu M) increased the cell viability (79.74 +/- 2.02 vs 90.98 +/- 2.66%, P < 0.01), improved proliferation (EdU-positive cells, 26.42 +/- 1.12 vs 32.05 +/- 0.78%, P < 0.01), decreased accumulation of intracellular reactive oxygen species (ROS, 1.68 +/- 0.05 vs 1.29 +/- 0.06, P < 0.01), stabilized mitochondrial-membrane potential (MMP, 8.98 +/- 1.40 vs 2.29 +/- 0.76, P < 0.001), and prevented apoptosis induced by DON (13.91 +/- 1.20 vs 6.83 +/- 0.52%, P < 0.01). RES activated the Nrf2 signaling pathway, and transfection with Nrf2 siRNA abrogated the protection of RES against DON-induced cytotoxicity, accumulation of intracellular ROS, and mitochondria dependent apoptosis. Collectively, RES protects IPEC-J2 cells against DON-induced damage at least partly via the Nrf2 signaling pathway.
- 相关文献
作者其他论文 更多>>
-
Baicalein inhibits PRRSV through direct binding, targeting EGFR, and enhancing immune response
作者：Wu, Jing;Lu, Qi;Hou, Jing;Qiu, Yueqin;Tian, Min;Wang, Li;Gao, Kaiguo;Yang, Xuefen;Jiang, Zongyong;Wu, Jing;Lu, Qi;Hou, Jing;Qiu, Yueqin;Tian, Min;Wang, Li;Gao, Kaiguo;Yang, Xuefen;Jiang, Zongyong;Wu, Jing;Lu, Qi;Hou, Jing;Qiu, Yueqin;Tian, Min;Wang, Li;Gao, Kaiguo;Yang, Xuefen;Jiang, Zongyong;Wu, Jing;Lu, Qi;Hou, Jing;Qiu, Yueqin;Tian, Min;Wang, Li;Gao, Kaiguo;Yang, Xuefen;Jiang, Zongyong
关键词：Piglets; PRRSV; baicalein; EGFR; MD simulation
-
Effect of Gardeniae Fructus Powder on Growth Performance, Antioxidant Capacity, Intestinal Barrier Function, and Colonic Microbiota of Weaned Piglets
作者：Liu, Shilong;Ma, Ming;Guo, Shining;Liu, Shilong;Tian, Min;Qiu, Yueqin;Tang, Jiaxi;Hou, Jing;Lu, Qi;Tian, Chaoyang;Ye, Guohao;Wang, Li;Gao, Kaiguo;Jiang, Zongyong;Yang, Xuefen;Liu, Shilong;Tian, Min;Qiu, Yueqin;Tang, Jiaxi;Hou, Jing;Lu, Qi;Tian, Chaoyang;Ye, Guohao;Wang, Li;Gao, Kaiguo;Jiang, Zongyong;Yang, Xuefen;Liu, Shilong;Tian, Min;Qiu, Yueqin;Tang, Jiaxi;Hou, Jing;Lu, Qi;Tian, Chaoyang;Ye, Guohao;Wang, Li;Gao, Kaiguo;Jiang, Zongyong;Yang, Xuefen;Liu, Shilong;Tian, Min;Qiu, Yueqin;Tang, Jiaxi;Hou, Jing;Lu, Qi;Tian, Chaoyang;Ye, Guohao;Wang, Li;Gao, Kaiguo;Jiang, Zongyong;Yang, Xuefen
关键词：piglet; Gardeniae Fructus; antioxidant capacity; intestinal barrier function; colonic microbiota
-
The Antinutritional Factors and Technological Processing of Sorghum and Its Application in Pig Production
作者：Zhang, Jianjian;Li, Ping;Yang, Xuefen;Wang, Li
关键词：sorghum; antinutritional factors; nutrient value; pig
-
Dietary bile acid supplementation improves the intestinal health and growth performance of piglets partially through the FXR/AQPs pathway
作者：Zhang, Beibei;Tian, Min;Yang, Yahui;Qiu, Yueqin;Wang, Li;Xiao, Hao;Zhu, Xiaoping;Yang, Xuefen;Jiang, Zongyong;Tian, Min;Qiu, Yueqin;Wang, Li;Xiao, Hao;Zhu, Xiaoping;Yang, Xuefen;Jiang, Zongyong;Tian, Min;Qiu, Yueqin;Wang, Li;Xiao, Hao;Zhu, Xiaoping;Yang, Xuefen;Jiang, Zongyong;Tian, Min;Qiu, Yueqin;Wang, Li;Xiao, Hao;Zhu, Xiaoping;Yang, Xuefen;Jiang, Zongyong;Qin, Limei
关键词：Bile acid; Piglet; Growth performance; Gut health; FXR
-
Effects of Dietary Ratio of Insoluble Fiber to Soluble Fiber on Reproductive Performance, Biochemical Parameters, and Fecal Microbial Composition of Gestating Sows
作者：Wen, Xiaolu;Wu, Qiwen;Gao, Kaiguo;Yang, Xuefen;Xiao, Hao;Jiang, Zongyong;Wang, Li;Wen, Xiaolu;Wu, Qiwen;Gao, Kaiguo;Yang, Xuefen;Xiao, Hao;Jiang, Zongyong;Wang, Li;Wen, Xiaolu;Wu, Qiwen;Gao, Kaiguo;Yang, Xuefen;Xiao, Hao;Jiang, Zongyong;Wang, Li;Wen, Xiaolu;Wu, Qiwen;Gao, Kaiguo;Yang, Xuefen;Xiao, Hao;Jiang, Zongyong;Wang, Li
关键词：sow; soluble fiber; insoluble fiber; fecal microbial; reproductive performance
-
Preliminary Research on Dietary Supplementation of Potassium Magnesium Sulphate on Transport Stress in Finishing Pigs Prior to Slaughter
作者：Cui, Bailei;Xiong, Yunxia;Wen, Xiaolu;Wu, Shengnan;Xiao, Hao;Cao, Shuting;Jiang, Zongyong;Wang, Li;Hu, Shenglan;Cui, Bailei;Huang, Yi
关键词：potassium magnesium sulphate; transport stress; finishing pigs; meat quality; stress response
-
Impact of Low-Dose Amino Acid-Chelated Trace Minerals on Performance, Antioxidant Capacity, and Fecal Excretion in Growing-Finishing Pigs
作者：Xiong, Yunxia;Li, Yaojie;Wu, Qiwen;Xiao, Huaqin;Cao, Shuting;Yang, Xuefen;Gao, Kaiguo;Jiang, Zongyong;Hu, Shenglan;Wang, Li;Zhao, Fei
关键词：amino acid-chelated trace minerals; meat quality; antioxidant capacity; fecal excretion; growing-finishing pigs
