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Dynamic Change of Gut Microbiota During Porcine Epidemic Diarrhea Virus Infection in Suckling Piglets

文献类型： 外文期刊

作者： Huang, Anni 1 ; Cai, Rujian 2 ; Wang, Qun 1 ; Shi, Lei 5 ; Li, Chunling 2 ; Yan, He 1 ;

作者机构： 1.South China Univ Technol, Sch Food Sci & Engn, Guangzhou, Guangdong, Peoples R China

2.Guangdong Acad Agr Sci, Inst Anim Hlth, Guangzhou, Guangdong, Peoples R China

3.Guangdong Prov Key Lab Livestock Dis Prevent, Guangzhou, Guangdong, Peoples R China

4.Guangdong Open Lab Vet Publ Hlth, Guangzhou, Guangdong, Peoples R China

5.Jinan Univ, Inst Food Safety & Nutr, Guangzhou, Guangdong, Peoples R China

关键词： gut microbiota; PEDV infection; suckling piglets; SCFA-producing bacteria; core microbiota

期刊名称：FRONTIERS IN MICROBIOLOGY （ 影响因子：5.64； 五年影响因子：6.32 ）

ISSN： 1664-302X

年卷期： 2019 年 10 卷

页码：

收录情况： SCI

摘要： Porcine epidemic diarrhea (PED) is a disease that has a devastating effect on livestock. Currently, most studies are focused on comparing gut microbiota of healthy piglets and piglets with PED, resulting in gut microbial populations related to dynamic change in diarrhea! piglets being poorly understood. The current study analyzed the characteristics of gut microbiota in porcine epidemic diarrhea virus (PEDV)-infected piglets during the suckling transition stage. Fresh fecal samples were collected from 1 to 3-weekold healthy piglets (n = 20) and PEDV infected piglets (n = 18) from the same swine farm. Total DNA was extracted from each sample and the V3-V4 hypervariable region of the 16S rRNA gene was amplified and sequenced using the Illumina MiSeq platform. Statistically significant differences were observed in bacterial diversity and richness between the healthy and diarrheal piglets. Principal coordinates analysis (PCoA) showed structural segregation between diseased and healthy groups, as well as among 3 different age groups. The abundance of Escherichia-Shigella, Enterococcus, Fusobacterium, and Veillonella increased due to dysbiosis induced by PEDV infection. Notably, there was a remarkable age-related increase in Fusobacterium and Veillonella in diarrheal piglets. Certain SOFA-producing bacteria, such as Rum inococcaceae_UCG-002, Butyricimonas, and Alistipes, were shared by all healthy piglets, but were not identified in various age groups of diarrheal piglets. In addition, significant differences were observed between clusters of orthologous groups (COG) functional categories of healthy and PEDV-infected piglets. Our findings demonstrated that PEDV infection caused severe perturbations in porcine gut microbiota. Therefore, regulating gut microbiota in an age-related manner may be a promising method for the prevention or treatment of PEDV.

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