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Engineering T7 bacteriophage as a potential DNA vaccine targeting delivery vector

文献类型： 外文期刊

作者： Xu, Hai 1 ; Bao, Xi 1 ; Wang, Yiwei 1 ; Xu, Yue 1 ; Deng, Bihua 1 ; Lu, Yu 1 ; Hou, Jibo 1 ;

作者机构： 1.Jiangsu Acad Agr Sci, Inst Vet Immunol & Engn, Nanjing 210014, Jiangsu, Peoples R China

2.Jiangsu Coinnovat Ctr Prevent & Control Important, Yangzhou 225009, Jiangsu, Peoples R China

关键词： Bacteriophage T7; Targeting delivery; Eukaryotic expression; Vaccine

期刊名称：VIROLOGY JOURNAL （ 影响因子：4.099； 五年影响因子：3.719 ）

ISSN： 1743-422X

年卷期： 2018 年 15 卷

页码：

收录情况： SCI

摘要： Background: DNA delivery with bacteriophage by surface-displayed mammalian cell penetrating peptides has been reported. Although, various phages have been used to facilitate DNA transfer by surface displaying the protein transduction domain of human immunodeficiency virus type 1 Tat protein (Tat peptide), no similar study has been conducted using T7 phage. Methods: In this study, we engineeredT7 phage as a DNA targeting delivery vector to facilitate cellular internalization. We constructed recombinant T7 phages that displayed Tat peptide on their surface and carried eukaryotic expression box (EEB) as a part of their genomes (T7-EEB-Tat). Results: We demonstrated that T7 phage harboring foreign gene insertion had packaged into infective progeny phage particles. Moreover, when mammalian cells that were briefly exposed to T7-EEB-Tat, expressed a significant higher level of the marker gene with the control cells infected with the wide type phage without displaying Tat peptides. Conclusion: These data suggested that the potential of T7 phage as an effective delivery vector for DNA vaccine transfer.

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