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Knockdown of UGT352A5 decreases the thiamethoxam resistance in Bemisia tabaci (Hemiptera: Gennadius)

文献类型： 外文期刊

作者： Du, Tianhua 1 ; Fu, Buli 1 ; Wei, Xuegao 1 ; Yin, Cheng 2 ; Yang, Jing 2 ; Huang, Mingjiao 2 ; Liang, Jinjin 2 ; Gong, Pe 1 ;

作者机构： 1.Yangtze Univ, Coll Agr, Hubei Engn Technol Ctr Pest Forewarning & Managem, Jingzhou 434025, Hubei, Peoples R China

2.Chinese Acad Agr Sci, Inst Vegetables & Flowers, Dept Plant Protect, Beijing 100081, Peoples R China

3.Chinese Acad Trop Agr Sci, Minist Agr & Rural Affairs, Key Lab Integrated Pest Management Trop Crops, Environm & Plant Protect Inst, Haikou 571101, Hainan, Peoples R China

关键词： UDP-glycosyltransferases; Bemisia tabaci; Thiamethoxam resistance

期刊名称：INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES （ 影响因子：6.953； 五年影响因子：6.737 ）

ISSN： 0141-8130

年卷期： 2021 年 186 卷

页码：

收录情况： SCI

摘要： Uridine diphosphate (UDP)-glycosyltransferases (UGTs), which are major phase II detoxification enzymes, have been implicated in the glycosylation of lipophilic endobiotics and xenobiotics and thus potentially lead to the evolution of insecticide resistance. In this study, we identified and cloned two putative UGT genes from transcriptome data which are named UGT352A4 and UGT352A5. As demonstrated by qRT-PCR, two UGT genes were over-expressed in the thiamethoxam-resistant (THQR) strain relative to the susceptible (THQS) strain. Moreover, the induction experiment revealed that the expression of the UGT352A5 gene was significantly increased following exposure to thiamethoxam in the THQR strain. Furthermore, the expression of both UGT352A4 and UGT352A5 was downregulated after RNA interference, whereas only the silencing of UGT352A5 resulted in a noticeable increase in the mortality of THQR adults. Our results represent the first line of evidence showing that UGT352A5 might be responsible for conferring thiamethoxam resistance in B. tabaci. The results will be shed new insights for obtaining a better understanding of the role of UGTs in the evolution of insecticide resistance and developing new insect resistance management tactics within the sustainable integrated pest management framework.

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