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Repressible Transgenic Sterilization in Channel Catfish, Ictalurus punctatus, by Knockdown of Primordial Germ Cell Genes with Copper-Sensitive Constructs

文献类型： 外文期刊

作者： Li, Hanbo 1 ; Su, Baofeng 1 ; Qin, Guyu 1 ; Ye, Zhi 1 ; Elaswad, Ahmed 1 ; Alsaqufi, Ahmed 1 ; Perera, Dayan A. 1 ; Qin, 1 ;

作者机构： 1.Auburn Univ, Sch Fisheries Aquaculture & Aquat Sci, Auburn, AL 36849 USA

2.Chinese Acad Fisheries Sci, Heilongjiang River Fisheries Res Inst, Natl & Local United Engn Lab Freshwater Fish Bree, Harbin 150070, Heilongjiang, Peoples R China

3.Suez Canal Univ, Dept Anim Wealth Dev, Fac Vet Med, Ismailia 41522, Egypt

4.King Faisal Univ, Dept Aquaculture & Anim Prod, Al Hasa 31982, Saudi Arabia

5.West Virginia State Univ, Gus R Douglass Inst, Corp Res & Dev, Institute, WV 25112 USA

6.Ocean Univ China, Key Lab Marine Genet & Breeding, Minist Educ, Qingdao 266003, Peoples R China

7.ICAR Res Complex NEH Reg, Umiam 793103, Meghalaya, India

关键词： Transgenic sterilization; Repressible by copper; PGC migration; RNAi

期刊名称：MARINE BIOTECHNOLOGY （ 影响因子：3.619； 五年影响因子：3.322 ）

ISSN： 1436-2228

年卷期： 2018 年 20 卷 3 期

页码：

收录情况： SCI

摘要： Repressible knockdown approaches were investigated to manipulate for transgenic sterilization in channel catfish, Ictalurus punctatus. Two primordial germ cell (PGC) marker genes, nanos and dead end, were targeted for knockdown and an off-target gene, vasa, was monitored. Two potentially copper-sensitive repressible promoters, yeast ctr3 (M) and ctr3-reduced (Mctr), were coupled with four knockdown strategies separately including: ds-sh RNA targeting the 5' end (N1) or 3' end (N2) of channel catfish nanos, full-length cDNA sequence of channel catfish nanos for overexpression (cDNA), and ds-sh RNA-targeting channel catfish dead end (DND). Each construct had an untreated group and treated group with copper sulfate as the repressor compound. Spawning rates of full-sibling P-1 fish exposed or not exposed to the constructs as treated and untreated embryos were 85 and 54%, respectively, indicating potential sterilization of fish and repression of the constructs. In F-1 fish, mRNA expressions of PGC marker genes for most constructs were downregulated in the untreated group and the knockdown was repressed in the treated group. Gonad development in transgenic, untreated F-1 channel catfish was reduced compared to non-transgenic fish for MctrN2, MN1, MN2, and MDND. For 3-year-old adults, gonad size in the transgenic untreated group was 93.4% smaller than the non-transgenic group for females and 92.3% for males. However, mean body weight of transgenic females (781.8 g) and males (883.8 g) was smaller than of non-transgenic counterparts (984.2 and 1254.3 g) at 3 years of age, a 25.8 and 41.9% difference for females and males, respectively. The results indicate that repressible transgenic sterilization is feasible for reproductive control of fish, but negative pleiotropic effects can result.

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