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Generation of epidermal growth factor and green fluorescent protein coexpressing Lactobacillus acidophilus and its effect on intestinal histology improvement in mature LPS-challenged mice

文献类型： 外文期刊

作者： Lv, Yang 1 ; Kang, Ping 1 ; Zhang, Lin 2 ; Li, Xueni 1 ; Wang, Lei 1 ; Zhao, Di 1 ; Yi, Dan 1 ; Ding, Binying 1 ; Chen, Hong 1 ;

作者机构： 1.Wuhan Polytech Univ, Hubei Key Lab Anim Nutr & Feed Sci, Hubei Collaborat Innovat Ctr Anim Nutr & Feed Saf, Wuhan 430023, Hubei, Peoples R China

2.Huazhong Agr Univ, Coll Fisheries, Wuhan 430070, Hubei, Peoples R China

3.Chinese Acad Fishery Sci, Yangtze River Fisheries Res Inst, Wuhan 430070, Hubei, Peoples R China

关键词： epidermal growth factor; Lactobacillus acidophilus; intestinal histology; LPS-challenged mice

期刊名称：THAI JOURNAL OF VETERINARY MEDICINE （ 影响因子：0.281； 五年影响因子：0.465 ）

ISSN： 0125-6491

年卷期： 2018 年 48 卷 2 期

页码：

收录情况： SCI

摘要： The aim of the present study was to construct a recombinant strain by using the probiotics Lactobacillus acidophilus expressing the epidermal growth factor (EGF), which can improve intestinal morphology, and to test its effect on the intestinal histology in LPS (lipopolysaccharide)-challenged mice. The EGF gene was cloned into the shuttle vector pSET4s and then transformed into the L. acidophilus MG6243 strain by electroporation. Two recombinant strains were confirmed by SDS-PAGE and named Delta MG6243-1 and Delta MG6243-2. Sixty mice were randomly assigned to six treatments: 1) non-challenged control, 2) LPS-challenged control, 3) LPS-challenged + 200 mu l MG6243, 4) LPSchallenged + 200 mu l Delta MG6243-1, 5) LPS-challenged + 200 mu l Delta MG6243-1 + inducer IPTG, and 6) LPS-challenged + 200 mu l Delta MG6243-2. There were ten replicates for each treatment, and each replicate had one mouse. On d 15, the LPS-challenged mice were injected intraperitoneally with LPS at 10 mg/kg BW, while the non-challenged mice were injected with 0.9% NaCl solution. Three hours after the challenge, all mice were killed. Results showed that both Delta MG6243-1 and Delta MG6243-2 could increase villus height in the duodenum, ileum and jejunum (P<0.05). In addition, the recombinant L. acidophilus decreased the crypt depth in the duodenum and ileum (P<0.05). These results indicated that the recombinant L. acidophilus could effectively improve the intestinal histology in LPS-challenged mice.

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