文献类型： 外文期刊
作者： Tao, Yajun 1 ; Wang, Jun 2 ; Miao, Jun 1 ; Chen, Jie 1 ; Wu, Shujun 3 ; Zhu, Jinyan 1 ; Zhang, Dongping 1 ; Gu, Houwen 1 ; C 1 ;
作者机构： 1.Yangzhou Univ, Jiangsu Key Lab Crop Genet & Physiol, Coinnovat Ctr Modern Prod Technol Grain Crops, Key Lab Plant Funct Genom,Minist Educ,Jiangsu Key, Yangzhou 225009, Jiangsu, Peoples R China
2.Jiangsu Acad Agr Sci, Inst Food Crops, Nanjing 210014, Peoples R China
3.Shanghai Acad Agr Sci, Shanghai 201403, Peoples R China
期刊名称：PLANT PHYSIOLOGY （ 影响因子：8.34； 五年影响因子：8.972 ）
ISSN： 0032-0889
年卷期： 2018 年 178 卷 4 期
页码：
收录情况： SCI
摘要： Polyamines, including putrescine, spermidine, and spermine, play essential roles in a wide variety of prokaryotic and eukaryotic organisms. Rice (Oryza sativa) contains four putative spermidine/spermine synthase (SPMS)-encoding genes (OsSPMS1, OsSPMS2, OsSPMS3, and OsACAULIS5), but none have been functionally characterized. In this study, we used a reverse genetic strategy to investigate the biological function of OsSPMS1. We generated several homozygous RNA interference (RNAi) and overexpression (OE) lines of OsSPMS1. Phenotypic analysis indicated that OsSPMS1 negatively regulates seed germination, grain size, and grain yield per plant. The ratio of spermine to spermidine was significantly lower in the RNAi lines and considerably higher in the OE lines than in the wild type, suggesting that OsSPMS1 may function as a SPMS. S-Adenosyl-L-methionine is a common precursor of polyamines and ethylene biosynthesis. The 1-aminocyclopropane-1-carboxylic acid (ACC) and ethylene contents in seeds increased significantly in RNAi lines and decreased in OE lines, respectively, compared with the wild type. Additionally, the reduced germination rates and growth defects of OE lines could be rescued with ACC treatment. These data suggest that OsSPMS1 affects ethylene synthesis and may regulate seed germination and plant growth by affecting the ACC and ethylene pathways. Most importantly, an OsSPMS1 knockout mutant showed an increase in grain yield per plant in a high-yield variety, Suken118, suggesting that OsSPMS1 is an important target for yield enhancement in rice.
- 相关文献
作者其他论文 更多>>
-
Generation of new rice germplasms with low amylose content by CRISPR/CAS9-targeted mutagenesis of the FLOURY ENDOSPERM 2 gene
作者：Song, Xiaohong;Li, Bin;Tan, Xiaoli;Li, Yulong;Yang, Jie;Song, Xiaohong;Chen, Zhihui;Du, Xi;Li, Bin;Fei, Yunyan;Tao, Yajun;Wang, Fangquan;Xu, Yang;Li, Wenqi;Wang, Jun;Liang, Guohua;Zhou, Yong;Yang, Jie;Chen, Zhihui;Du, Xi;Fei, Yunyan;Tao, Yajun;Wang, Fangquan;Xu, Yang;Li, Wenqi;Wang, Jun;Liang, Guohua;Zhou, Yong;Yang, Jie
关键词：FLOURY ENDOSPERM 2; amylose content (AC); eating and cooking quality (ECQ); japonica rice; CRISPR; Cas9; genome editing
-
Development of high internal phase emulsions with noncovalent crosslink of soy protein isolate and tannic acid: Mechanism and application for 3D printing
作者：Hu, Wenyi;Chen, Chunli;Wang, Ying;Li, Jianlin;Li, Weiwei;He, Weiwei;He, Zhiyong;Chen, Jie;Li, Zongan
关键词：Soy protein; Tannic acid; High internal phase emulsion; Noncovalent interaction; 3D printing
-
Fertilization restructures nematode assemblages by modifying soil pH in croplands of Northeast China
作者：Li, Chunlin;Xie, Zhonglei;Wang, Jun;Yang, Zhenming;Wang, Xuefeng;Chen, Baoyu;Wang, Lichun
关键词：long-term fertilization; soil type; soil acidification; nematode assemblage; Northeast China
-
Increased N2O emission due to paddy soil drainage is regulated by carbon and nitrogen availability
作者：Wu, Lei;Wang, Jun;Zhang, Wenju;Xu, Minggang;Tang, Shuirong;Hu, Ronggui;Duan, Pengpeng;Xu, Cong
关键词：N2O emission; Paddy soil drainage; Nitrifying and denitrifying genes; (nirK plus nirS)/nosZ ratio
-
Alginate oligosaccharides protect against fumonisin B1-induced intestinal damage via promoting gut microbiota homeostasis
作者：Li, Tiantian;Wang, Jun;Yin, Peng;Liu, Hujun;Sun, Changpo;Huang, Shimeng;Huang, Shimeng;Sun, Changpo
关键词：Mycotoxin; Prebiotics; Anti-apoptotic; Anti-inflammatory; Gut microbiome; Gut dysbiosis
-
The microbial-driven C dynamics within soil aggregates in walnut orchards of different ages based on microbial biomarkers analysis
作者：Luan, Haoan;Zhang, Xuemei;Liu, Yang;Guo, Suping;Qi, Guohui;Liu, Yingru;Huang, Shaohui;Chen, Jie;Guo, Tengfei
关键词：Walnut plantation ages; Soil aggregates; Microbial characteristics; Soil C dynamics
-
Importance of hydrogen sulfide as the molecular basis of heterosis in hybrid Brassica napus: A case study in salinity response
作者：Cheng, Pengfei;Zhang, Yihua;Wang, Jun;Shen, Wenbiao;Guan, Rongzhan;Pu, Huiming
关键词：Heterosis; Hydrogen sulfide; Salt stress; Brassica napus
