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Protective role of antifreeze proteins on sterlet (Acipenser ruthenus) sperm during cryopreservation

文献类型： 外文期刊

作者： Xin, Miaomiao 1 ; Sterba, Jan 3 ; Shaliutina-Kolesova, Anna 1 ; Dzyuba, Borys 1 ; Lieskovska, Jaroslava 3 ; Boryshp 1 ;

作者机构： 1.Univ South Bohemia Ceske Budejovice, Fac Fisheries & Protect Waters, South Bohemian Res Ctr Aquaculture & Biodivers Hy, Res Inst Fish Culture & Hydrobiol, Zatisi 728-2, Vodnany 38925, Czech Republic

2.Chinese Acad Fishery Sci, Sinoczech Joint Lab Fish Conservat & Biotechnol, Key Lab Freshwater Biodivers Conservat, Minist Agr China,Yangtze River Fisheries Res Inst, Wuhan, Hubei, Peoples R China

3.Univ South Bohemia Ceske Budejovice, Fac Sci, Branisovska 1760, Ceske Budejovice 37005, Czech Republic

4.Czech Acad Sci, Biol Ctr, Inst Parasitol, Branisovska 31, Ceske Budejovice 37005, Czech Republic

5.Noakhali Sci & Technol Univ, Dept Oceanog, Noakhali 3814, Bangladesh

关键词： Antifreeze proteins; Cryopreservation; Membrane integrity; Motility rate; Sperm quality

期刊名称：FISH PHYSIOLOGY AND BIOCHEMISTRY （ 影响因子：2.794； 五年影响因子：2.876 ）

ISSN： 0920-1742

年卷期： 2018 年 44 卷 6 期

页码：

收录情况： SCI

摘要： The loss of sperm quality in sterlet (Acipenser ruthenus) due to freeze-thaw process in cryopreservation was investigated in the present study. Two antifreeze proteins (AFPI or AFPIII) were used at different concentrations of 0.1, 1, 10, and 100g/mL. We compared motility, curvilinear velocity, and plasma membrane integrity of fresh, cryopreserved sperm, and sperm cryopreserved in the presence of antifreeze proteins. Fresh sperm (control) had 85 +/- 4% motility and 160 +/- 2m/s curvilinear velocity, respectively. After cryopreservation, the motility of frozen-thawed sperm without addition of antifreeze proteins significantly decreased (44 +/- 9%), compared to the control. The highest motility of frozen-thawed sperm was obtained in cryopreserved sperm with addition of 1g/mL of AFPIII (58 +/- 14%). No significant differences were observed in curvilinear velocity between fresh sperm and cryopreserved sperm with/without addition of AFPI or AFPIII. The flow cytometry analysis revealed that fresh sperm contained 94.5 +/- 6% live cells, while the cryopreserved sperm only contained 26.6 +/- 14% live cells. Supplementation of antifreeze proteins has significantly improved the percentage of live cells in frozen-thawed sperm, except 0.1g/ml of AFPI group. No significant difference in percentage of live cells was detected in the sperm cryopreserved with 10g/mL of AFPI or AFPIII, compared to fresh sperm. Thus, addition of antifreeze proteins to cryopreservation medium could be considered to improve the post-thawed sperm quality of sterlet.

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