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Transcriptomic Characterization of the South American Freshwater Stingray Potamotrygon motoro Venom Apparatus

文献类型： 外文期刊

作者： Silva, Filipe 1 ; Huang, Yu 3 ; Yang, Vitor 1 ; Mu, Xidong 5 ; Shi, Qiong 3 ; Antunes, Agostinho 1 ;

作者机构： 1.Univ Porto, Interdisciplinary Ctr Marine & Environm Res, CIIMAR CIMAR, Av Gen Norton de Matos S-N, P-4450208 Porto, Portugal

2.Univ Porto, Fac Sci, Dept Biol, Rua Campo Alegre S-N, P-4169007 Porto, Portugal

3.BGI Acad Marine Sci, Shenzhen Key Lab Marine Genom, Guangdong Prov Key Lab Mol Breeding Marine Econ A, Shenzhen 518083, Peoples R China

4.Univ Chinese Acad Sci, BGI Educ Ctr, Shenzhen 518083, Peoples R China

5.Chinese Acad Fishery Sci, Guangdong Engn Technol Res Ctr Adv Recreat Fisher, Pearl River Fisheries Res Inst, Minist Agr,Key Lab Recreat Fisheries, Guangzhou 510380, Guangdong, Peoples R China

关键词： venom; transcriptomics; stingray; hyaluronidase; next-generation sequencing; selective pressure

期刊名称：TOXINS （ 影响因子：4.546； 五年影响因子：4.8 ）

ISSN： 2072-6651

年卷期： 2018 年 10 卷 12 期

页码：

收录情况： SCI

摘要： Venomous animals are found through a wide taxonomic range including cartilaginous fish such as the freshwater stingray Potamotrygon motoro occurring in South America, which can injure people and cause venom-related symptoms. Ensuring the efficacy of drug development to treat stingray injuries can be assisted by the knowledge of the venom composition. Here we performed a detailed transcriptomic characterization of the venom gland of the South American freshwater stingray Potamotrygon motoro. The transcripts retrieved showed 418 hits to venom components (comparably to 426 and 396 hits in other two Potamotrygon species), with high expression levels of hyaluronidase, cystatin and calglandulin along with hits uniquely found in P. motoro such as DELTA-alicitoxin-Pse1b, Augerpeptide hhe53 and PI-actitoxin-Aeq3a. We also identified undescribed molecules with extremely high expression values with sequence similarity to the SE-cephalotoxin and Rapunzel genes. Comparative analyses showed that despite being closely related, there may be significant variation among the venoms of freshwater stingrays, highlighting the importance of considering elicit care in handling different envenomation cases. Since hyaluronidase represents a major component of fish venom, we have performed phylogenetic and selective pressure analyses of this gene/protein across all fish with the available information. Results indicated an independent recruitment of the hyaluronidase into the stingray venom relative to that of venomous bony fish. The hyaluronidase residues were found to be mostly under negative selection, but 18 sites showed evidence of diversifying positive selection (P < 0.05). Our data provides new insight into stingray venom variation, composition, and selective pressure in hyaluronidase.

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