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Application of high-resolution melting curve analysis for identification of Muscovy duck parvovirus and goose parvovirus

文献类型： 外文期刊

作者： Dong, Jiawen 4 ; Bingga, Gali 2 ; Sun, Minhua 1 ; Li, Linlin 1 ; Liu, Zhicheng 1 ; Zhang, Chunhong 1 ; Guo, Pengju 3 ; Hu 1 ;

作者机构： 1.Guangdong Acad Agr Sci, Inst Anim Hlth, Wushan Rd, Guangzhou 510640, Guangdong, Peoples R China

2.Inner Mongolia Agr Univ, Vocat & Tech Coll, Hohhot 014109, Inner Mongolia, Peoples R China

3.Guangdong Lab Anim Monitoring Inst, Guangdong Key Lab Lab Anim, Guangzhou 510633, Guangdong, Peoples R China

4.Key Lab Livestock Dis Prevent Guangdong Prov, Guangzhou 510640, Guangdong, Peoples R China

5.Minist Agr, Sci Observat & Expt Stn Vet Drugs & Diagnost Tech, Guangzhou 510640, Peoples R China

6.Fujian Acad Agr Sci, Inst Anim Husb & Vet Med, Fuzhou 350013, Fujian, Peoples R China

关键词： Muscovy duck parvovirus; Goose parvovirus; VP3 gene; HRM

期刊名称：JOURNAL OF VIROLOGICAL METHODS （ 影响因子：2.014； 五年影响因子：2.001 ）

ISSN： 0166-0934

年卷期： 2019 年 266 卷

页码：

收录情况： SCI

摘要： This study reports the findings of a high-resolution melting (HRM) curve analysis combined with PCR technique (PCR-HRM) to differentiate between Muscovy duck parvovirus (MDPV) and goose parvovirus (GPV). A degenerate primer set was designed based on the VP3 gene of MDPV and GPV. The PCR HRM assay was able to discriminate between MDPVs and GPVs by differences in melting curve shapes and melting temperatures. A total of forty-five clinical samples, passaged in the allantoic cavity of Muscovy duck eggs, were detected by the PCR-HRM assay. Among the 12 positive samples, two were identified as MDPV and two as GPV with high genotype confidence percentage (GCP) values. Seven positive samples had low GCP values for the melting curve analysis and were identified as co-infection samples. One of the 12 positive samples, designed GDNX strain, was identified as a variant strain with a divergent melting curve profile. To assess the capability of PCR-HRM assay to distinguish MDPV and GPV, fifty-two field samples were collected and examined. Seven samples were positive for MDPV and/or GPV. Thus, this developed assay was useful for discrimination of MDPVs and GPVs and can also be suitable for detecting co-infection samples.

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