MicroRNA-205 affects mouse granulosa cell apoptosis and estradiol synthesis by targeting CREB1
文献类型： 外文期刊
作者： Zhang, Pengju 1 ; Wang, Jun 2 ; Lang, Hongyan 1 ; Wang, Weixia 1 ; Liu, Xiaohui 1 ; Liu, Haiyan 1 ; Tan, Chengcheng 1 ; L 1 ;
作者机构： 1.Jilin Acad Agr Sci, Inst Anim Sci, 1363 Shengtai St, Changchun 130124, Jilin, Peoples R China
2.Jilin Agr Univ, Coll Anim Sci & Technol, Changchun, Jilin, Peoples R China
关键词： apoptosis; cyclic AMP response element (CRE)-binding protein 1 (CREB1); estradiol synthesis; follicular atresia; miR-205
期刊名称：JOURNAL OF CELLULAR BIOCHEMISTRY （ 影响因子：4.429； 五年影响因子：4.266 ）
ISSN： 0730-2312
年卷期： 2019 年 120 卷 5 期
页码：
收录情况： SCI
摘要： MicroRNA-205 (miR-205) is involved in various physiological and pathological processes, but its biological function in follicular atresia remains unclear. In this study, we investigated miR-205 expression in mouse granulosa cells (mGCs) and analyzed its functions in primary mGCs by performing a series of in vitro experiments. Quantitative real-time polymerase chain reaction showed that miR-205 expression was significantly higher in early atretic follicles and progressively atretic follicles than in healthy follicles. miR-205 overexpression in mGCs significantly promoted apoptosis and caspase-3/9 activities, as well as inhibited estrogen (E2) release and cytochrome P450 family 19 subfamily A polypeptide 1 (CYP19A1, a key gene in E2 production) expression. Bioinformatics and luciferase reporter assays revealed that the gene encoding cyclic AMP response element (CRE)-binding protein 1 (CREB1) was a direct target of miR-205 in mGCs. CREB1 upregulation partially rescued the effects of miR-205 on apoptosis, caspase-3/9 activities, E2 production, and CYP19A1 expression on mGCs. These results indicate that miR-205 might play an important role in ovarian follicular development and provide new insights into follicular atresia
- 相关文献
作者其他论文 更多>>
-
Relationship between the CUBN and the MIA3 gene copy number variation and growth traits in different cattle breeds
作者：Han, Yue;Liu, Jiwei;Zhang, Congcong;Sun, Ming;Li, Xuanyu;Liu, Hongliang;Li, Shengnan;Zhu, Yongchao;Li, Ruidong;Luo, Xiaotong;Zhao, Yumin;Wu, Jian;Han, Yue;Liu, Jiwei;Zhang, Congcong;Sun, Ming;Li, Xuanyu;Liu, Hongliang;Li, Shengnan;Zhu, Yongchao;Li, Ruidong;Luo, Xiaotong;Zhao, Yumin;Wu, Jian;Han, Yue;Liu, Jiwei;Zhang, Congcong;Sun, Ming;Li, Xuanyu;Liu, Hongliang;Li, Shengnan;Zhu, Yongchao;Li, Ruidong;Luo, Xiaotong;Zhao, Yumin;Wu, Jian;Han, Yue;Liu, Jiwei;Zhang, Congcong;Sun, Ming;Li, Xuanyu;Liu, Hongliang;Li, Shengnan;Zhu, Yongchao;Li, Ruidong;Luo, Xiaotong;Zhao, Yumin;Wu, Jian;Han, Yue;Liu, Jiwei;Zhang, Congcong;Sun, Ming;Li, Xuanyu;Liu, Hongliang;Li, Shengnan;Zhu, Yongchao;Li, Ruidong;Luo, Xiaotong;Zhao, Yumin;Wu, Jian;Han, Yue;Liu, Jiwei;Zhang, Congcong;Sun, Ming;Li, Xuanyu;Liu, Hongliang;Li, Shengnan;Zhu, Yongchao;Li, Ruidong;Luo, Xiaotong;Zhao, Yumin;Wu, Jian
关键词：Grassland red cattle; yanbian cattle; yan Yellow cattle; intrinsic factor-cobalamin receptor; MIA SH3 domain ER-derived factor 3; copy number variations
-
Multiple strategies association revealed functional candidate FASN gene for fatty acid composition in cattle
作者：Zhu, Bo;Wang, Tianzhen;Niu, Qunhao;Wang, Zezhao;Xu, Lei;Chen, Yan;Zhang, Lupei;Gao, Xue;Gao, Huijiang;Xu, Lingyang;Li, Junya;Zhu, Bo;Hay, El Hamidi;Xu, Lei;Cao, Yang;Zhao, Yumin;Cao, Yang;Zhao, Yumin
关键词：
-
GCF-DeepLabv3+: An Improved Segmentation Network for Maize Straw Plot Classification
作者：Liu, Yuanyuan;Zhang, Jiaxin;Liu, Yuanyuan;Zhang, Jiaxin;Wang, Yueyong;Wang, Jun;Luo, Yang;Sui, Pengxiang;Ren, Ying;Liu, Xiaodan
关键词：conservation tillage; DeepLabv3+; remote sensing; straw coverage; semantic segmentation
-
Long-read sequencing uncovers key regulatory genes involved in the differentiation of preadipocytes of Chinese red steppe cattle
作者：Fang, Wenwen;Li, Xuanyu;Liu, Yu;Cao, Yang;Xiao, Cheng;Li, Xin;Jin, Haiguo;Zhao, Yumin;Cao, Yang;Yang, Shaoying;Nanaei, Asadollahpour;Zhao, Yumin
关键词：ONT; Preadipocyte differentiation; Genes; AS; Chinese red steppe cattle
-
De novo genome assembly of Yanbian cattle using PacBio HiFi and Hi-C combined with RNA-seq data
作者：Fang, Wenwen;Cao, Yang;Liu, Yu;Wu, Jian;Miao, Lisheng;Xiao, Cheng;Li, Xin;Zhao, Yumin;Cao, Yang;Zhao, Yumin;Lv, Aihui
关键词：
-
Molecular patterns of matrix protein 1 (M1): A strong predictor of adaptive evolution in H9N2 avian influenza viruses
作者：Zhu, Yanting;Cong, Yulin;Sheng, Shouzhi;Liu, Chang;Jiang, Jianfeng;Li, Siqi;Gao, Chao;Zhao, Jinghui;Cong, Yanlong;Zhu, Yanting;Cong, Yulin;Sheng, Shouzhi;Liu, Chang;Jiang, Jianfeng;Li, Siqi;Gao, Chao;Zhao, Jinghui;Cong, Yanlong;Sun, Yixue;Zhang, Pengju
关键词：matrix protein M1; genetic pattern; virus replication; epidemiological dominance
-
Minimizing the potential risk of soil nitrogen loss through optimal fertilization practices in intensive agroecosystems
作者：Wang, Jun;Zhang, Lu;Xu, Minggang;Zhang, Wenju;Liu, Kailou;Zhou, Boku;Gao, Hongjun;Han, Xiaori;Liu, Shutang;Huang, Shaomin;Zhang, Aijun;Hua, Keke;Wang, Jidong;Hu, Hangwei
关键词：Gross nitrification; Gross N immobilization; Manure; Chemical fertilizer; Microbial abundance
