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Combined enzymatic hydrolysis and selective fermentation for green production of alginate oligosaccharides from Laminaria japonica

文献类型： 外文期刊

作者： Li, Shang-Yong 1 ; Wang, Zhi-Peng 2 ; Wang, Lin-Na 2 ; Peng, Ji-Xing 2 ; Wang, Ya-Nan 1 ; Han, Yan-Tao 1 ; Zhao, Shou- 1 ;

作者机构： 1.Qingdao Univ, Coll Basic Med, Dept Pharmacol, Qingdao 266071, Peoples R China

2.Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Key Lab Sustainable Dev Polar Fishery, Minist Agr & Rural Affairs, Qingdao 266071, Peoples R China

3.Qingdao Municipal Hosp, Cent Labs, Qingdao 266071, Shandong, Peoples R China

关键词： Alginate oligosaccharides; Laminaria japonica; Cellulase; Alginate lyase; Yarrowia lipolytica

期刊名称：BIORESOURCE TECHNOLOGY （ 影响因子：9.642； 五年影响因子：9.237 ）

ISSN： 0960-8524

年卷期： 2019 年 281 卷

页码：

收录情况： SCI

摘要： Alginate oligosaccharides (AOS) showed various biological activities. Traditional protocol for producing AOS was a multiple-step and high-pollution procedure. In this study, a rapid and efficient AOS producing method was developed directly from Laminaria japonica. Natural sun-dried L. japonica with a feed ratio of 1:7 (w/v) was pretreated using cellulase with a dry weight of 3%, for releasing the fermentable sugars (8.5 g/L glucose and 15.2 g/L mannitol). Then, the engineered yeast Yarrowia lipolytica strain with alginate lyase activity was grown using an algae-based medium. After fermentation for 72 h, glucose and mannitol were completely consumed, and 71.8 mM AOS was extracted from the fermentation supernatant. The degree of polymerization (DP) was ranging from 2 to 3. The recovery yield of AOS was about 91.7%. The purity of the extracted AOS was 92.6%. Overall, our work provided new insights for the development of green biotechnologies for oligosaccharide production from seaweed.

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