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Effects of Tea-Polysaccharide Conjugates and Metal Ions on Precipitate Formation by Epigallocatechin Gallate and Caffeine, the Key Components of Green Tea Infusion

文献类型: 外文期刊

作者: Chen, Xiaoqiang 1 ; Du, Yu 1 ; Wu, Long 1 ; Xie, Jianchun 2 ; Chen, Xueling 3 ; Hu, Binbin 4 ; Wu, Zhengqi 1 ; Yao, Qifen 1 ;

作者机构: 1.Hubei Univ Technol, Coll Bioengn & Food, Wuhan 430068, Hubei, Peoples R China

2.BTBU, Beijing Adv Innovat Ctr Food Nutr & Human Hlth, Beijing 100048, Peoples R China

3.Hubei Acad Agr Sci, Inst Farm Prod Proc & Nucl Agr Technol, Wuhan 430064, Hubei, Peoples R China

4.Hubei Prov Tea Deep Proc Engn Technol Res Ctr, Yichang 443000, Peoples R China

关键词: green tea infusion; precipitation; tea-polysaccharide conjugates; metal ions; epigallocatechin gallate (EGCG); caffeine

期刊名称:JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY ( 影响因子:5.279; 五年影响因子:5.269 )

ISSN: 0021-8561

年卷期: 2019 年 67 卷 13 期

页码:

收录情况: SCI

摘要: The mechanism of tea-infusion precipitation is not clear. Epigallocatechin gallate (EGCG) and caffeine (CAF) are the core components of green tea infusion precipitate. The critical precipitation concentration (C-CPC) of EGCG-CAF mixed aqueous solutions was assayed through a turbidity assay performed at 25 degrees C and verified by a differential-scanning calorimeter (DSC) for reliability. Different concentrations of the green tea polysaccharide conjugate fraction (gTPC-D), with a molecular weight of 2.32 x 10(5) Da, and of metal ions (K+, Mg2+, Ca2+, and Al3+) were respectively introduced into the mixed solution to study their effects on C-CPC. On the basis of the changed C-CPC, the clarification-precipitation phase diagrams of the mixed aqueous solutions were constructed. By calculating the integral area of the clarification phase, it was determined that the four metal ions and gTPC-D could accelerate precipitate formation with a dose-response relationship; among the metal ions, the effect of Al3+ is the strongest, followed by that of Ca2+.

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